本项目围绕心、肺经研究经络学说的经脉现象、经脉循行中体表—内脏之间、体表—体表之间的联系特点、规律及其生物学基础等关键问题。在“心、肺二经循经经脉现象的生物学特征”方面，已初步揭示健康者、心肺疾病患者心肺二经的微循环特性、热传输特性、代谢特性及电学特性，呈现相对特异性；在“心、肺经经脉循经现象的生物学机制”方面，已建立微透析—高效液相色谱—电喷雾串联四级杆质谱检测方法，发现大鼠DMNV核团中的神经递质含量与肺功能相关；在“心、肺经对心、肺功能的影响”方面，发现肺经五输穴对肺部疾病有特异性的治疗作用，心经五输穴对心脏疾病有特异性的治疗作用，电针改善心肌缺血小鼠心脏功能与α、β-AR表达有关；在“心、肺疾病的体表反应与心、肺经循行的相关性及机制研究”方面，发现心肺疾病模型大鼠体表神经源性反应分布与心肺经循行或同节段有较高一致性，心肺疾病患者出现的压痛点和局部皮肤异常改变与心肺经的循行部位存在着高度重合性；在“基于经脉“体表—内脏”联系的循经针刺效应的临床评价”方面，已制定针刺干预慢性持续性哮喘和慢性稳定型心绞痛的临床研究方案，并开展临床研究；在“基于经脉“体表—体表”联系的循经针刺效应的临床评价及其机制”方面，已制定针刺干预肩前痛和颈型颈椎病的临床研究方案，并开展临床研究。 This project focuses on key questions of the meridian theory,such as meridian phenomena,characteristics and rules underlying distributions and associations of meridians(meridian-viscera and site-to-site association),as well as the biological basis of meridians.Regarding biological characteristics of meridian phenomena for the heart and lung meridians,we have preliminary revealed the microcirculatory,thermal,metabolic and electrical characteristics of these two meridians in healthy adults and patients of cardiopulmonary diseases.Results indicate that meridian specificity exists.Regarding biological mechanism of meridian phenomena for these two meridians,detection methods of microdialysis sampling and high performance liquid chromatography-electrospray tandem mass spectrometry have been established.Results indicate that the content of neurotransmitter in the dorsal motor nucleus of the vagus has correlation with the lung function.Regarding effects of these two meridians on cardiopulmonary functions,results reveal five-shu acupoints of lung meridian have specific therapeutic effect on lung diseases.Five-shu acupoints of heart meridian have specific therapeutic effect on heart diseases and electroacupuncture can improve the heart function in myocardial ischemia mice by regulating expression of α and β-AR.Regarding correlations and mechanism investigations between peripheral responses of cardiopulmonary diseases and distributions of these two meridians,results indicate that distributions of neurogenic responses in rats of cardiopulmonary diseases is consistent with circulations of these two meridians or the the same segment.In patients of cardiopulmonary diseases,distributions of tender points and regional abnormal skin changes are also highly coincident with the circulation of these two meridians.Regarding clinical evaluations on the effect of acupuncture according to the meridian-viscera association theory,protocols of acupuncture on chronic persistent asthma and stable angina pectoris are built and trials are ongoing.Regarding the mechanism clinical evaluation on the effect of acupuncture based on the site-to-site association theory,protocols of acupuncture on anterior shoulder pain and cervical spondylosis are established and trials are ongoing.

本研究的围绕经络学说中的经脉现象、经脉循行中体表—内脏之间、体表—体表之间的联系特点、规律及其生物学基础等关键问题，以心经与肺经为切入点，采用动物实验结合临床验证的方法。在“心、肺二经循经经脉现象的生物学特征”方面，已开展第一轮检测，采用激光多普勒检测、红外热成像技术及近红外光谱技术检测了不同受试者对应的生物学特征。在“心、肺经经脉循经现象的生物学机制”方面，发现针刺可引起DMNV核团中的5-羟吲哚乙酸、氯化乙酰胆碱、天冬氨酸等神经递质的变化；在“心、肺经对心、肺功能的影响”方面，已开展心肺二经对心肺疾病相对特异性调节作用的临床和实验研究，发现针刺肺经五输穴对肺部疾病有特异性治疗作用；在“心、肺疾病的体表反应与心、肺经循行的相关性及机制研究”方面，已开展心肌缺血模型大鼠体表神经源性反应分布规律及痛阈值观察、支气管炎模型大鼠体表神经源性反应标记及分布规律和冠心病患者体表反应区分布规律及体表痛阈值观察研究；在“基于经脉“体表—内脏”联系的循经针刺效应的临床评价”方面，优化针刺干预慢性持续性哮喘和慢性稳定型心绞痛的临床研究方案，并开启了预实验；在“基于经脉“体表—体表”联系的循经针刺效应的临床评价及其机制”方面，优化针刺干预肩前痛和颈型颈椎病的临床研究方案，并开启了预实验。 This study focuses on the key issues of the meridian theory,such as the meridian phenomena,the characteristics and rules regarding the distribution and association of meridians(meridian-viscera association and surface-surface association),the biological basis of meridians.And the heart and lung meridians are chosen as the the entry point.We adopt animal experiments combined with clinical trials as research method.Regarding the biological characteristics for the meridian phenomena for heart and lung meridians,we have finished the first-round examination.Laser doppler flowmetry,infrared thermography and multi electrode impedance measurement have been used to detect the biological characteristics of meridian phenomena in different kinds of subjects.Regarding the biological mechanism of the meridian phenomena for heart and lung meridians,results have revealed that acupuncture could bring changes in neurotransmitters,such as 5-hydroxyindoleacetic acid,chlorinated acetylcholine and aspartic acid in DMNV nuclei.Regarding the effects of heart and lung meridians on heart and lung functions,clinical and experimental studies on the specific regulatory effects of heart and lung meridians on heart and lung diseases have been carried out.Relevant results have revealed that needling at five-shu points could have specific therapeutic effects on lung diseases.Regarding the mechanism for peripheral response of heart and lung diseases as well as the correlation of heart and lung meridians,research on the rules of peripheral response point distribution and examinations of pain threshold have been carried out in myocardial ischemia rats,bronchitis rats and coronary heart disease patients.Regarding the clinical evaluation of the effect of acupuncture based on the theory of meridian-viscera association,the protocols of acupuncture intervention on chronic persistent asthma and chronic stable angina pectoris have been optimized.Pilot study has started.Regarding the mechanism clinical evaluation of the effect of acupuncture based on the theory of surface-surface association,the protocols of acupuncture intervention on anterior shoulder pain and cervical spondylosis have been optimized.Pilot study has started.