项目来源

美国卫生和人类服务部基金(HHS)

项目主持人

未公开

项目受资助机构

UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON

项目编号

2R01HD017802-03

立项年度

1985

立项时间

未公开

研究期限

未知 / 未知

项目级别

国家级

受资助金额

未知

学科

未公开

学科代码

未公开

基金类别

未公开

关键词

未公开

参与者

STEINBERGER, ANNA

参与机构

EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT

项目标书摘要：Mammalian spermatogenesis is a hormone-dependent process regulated mainly by follicle-stimulating hormone (FSH) and testosterone (T). The effects of these hormones on germ cell (Gc) differentiation are believed to be indirect, mediated through the somatic Sertoli cells (Sc). Although T and/or FSH were shown to affect various Sc functions, including the secretion of many proteins (i.e., androgen binding protein, inhibin, transferrin, plasminogen activator), the mechanism by which the Sc mediates the hormonal effects on the Gc is not known. The overall objective of this proposal is to clarify the Sc-Gc interaction by further exploring a hypothesis that certain products which may be required by the Gc, are produced by the Sc and are transferred to the Gc during certain stages of their differentiation. The specific aims are: 1) to investigate the uptake of some prelabeled Sc products (peptides, proteins, glycoproteins and RNA) by the Gc at specific stages of differentiation, by using radioautography and isotope counting methods. During the initial grant period, we obtained evidence for high affinity, specific binding of androgen binding protein to the pachytene spermatocytes, and binding of transferrin to both pachytene spermatocytes and round spermatids, suggesting that some Sc products may be transferred to the Gc by a receptor-mediated mechanism; 2) to explore the effects of FSH and T on the secretion and transfer of the Sc products; 3) to identify the chemical nature of the transferred Sc products, not yet characterized, by using various immunologic (RIA, immunoprecipitation, immunofluorescence) and biochemical (two-dimensional gel electrophoresis, Western blots, HPLC, etc.) methods; 4) to examine if there is a correlation between the transfer of specific Sc products and Gc differentiation. These studies should provide additional information on the mechanism by which hormones regulate spermatogenesis and on the role of Sertoli cells in this process.