项目来源

德国科学基金(DFG)

项目主持人

Professor Dr. Markus Rodehutscord

项目受资助机构

未公开

立项年度

2017

立项时间

未公开

项目编号

322916021

项目级别

国家级

研究期限

未知 / 未知

受资助金额

未知

学科

Agriculture,Forestry and Veterinary Medicine

学科代码

未公开

基金类别

Research Units

关键词

未公开

参与者

Markus Rodehutscord

参与机构

-Universität Hohenheim-Institut für Nutztierwissenschaften-Fachgebiet Tierernährung

项目标书摘要：Our overall objective is to explore and understand the genetic and non-genetic variations in the formation of inositol phosphate derivatives and myo-inositol by endogenous phytases,as well as their relevance for phosphorus utilization,microbiome,and animal physiology in the fowl.In this Research Unit we test three overarching hypotheses:1.)phosphorus(P)utilization is driven by the bird’s genetic background and the active fraction of the intestinal microbiome,enabling targeted breeding for improved P utilization;2.)changes in microbiome,depending on age and feeding,contribute to formation of inositol phosphates and myo-inositol and subsequent physiological and immunological functions;3.)key physiological features can be identified to scale up the phenotyping of inositol phosphate and myo-inositol metabolism in larger populations.We use laying hens and Japanese quail as the animal models.In the laying hen experiments,we consider the age of the hens,their genetic background,and variations in dietary calcium(Ca)and P concentration as the experimental factors.We investigate phytic acid degradation products and phytase activity in the digestive tract,characterize the hen’s genetic background and the microbiota composition,study absorption and systemic effects of myo-inositol and their interrelationship with different mitochondrial haplotypes,other epithelial functions and smooth muscle contractility,and modulations of the gut-associated and systemic immune system.In the quail studies,samples of intestinal digesta and tissues from birds belonging to a well-defined family structure are investigated for relationships between P utilization and the intestinal microbiota.The data set,together with data obtained in preliminary work,is utilized in comprehensive QTL linkage and association analyses of P utilization,and investigated for functional putative candidate genes.The proposed Research Unit offers the unique opportunity to perform all these studies with the same animals of well-defined genetic background,and integrated bioinformatics analysis of all data.

Application Abstract: Our overall objective is to explore and understand the genetic and non-genetic variations in the formation of inositol phosphate derivatives and myo-inositol by endogenous phytases,as well as their relevance for phosphorus utilization,microbiome,and animal physiology in the fowl.In this Research Unit we test three overarching hypotheses:1.)phosphorus(P)utilization is driven by the bird’s genetic background and the active fraction of the intestinal microbiome,enabling targeted breeding for improved P utilization;2.)changes in microbiome,depending on age and feeding,contribute to formation of inositol phosphates and myo-inositol and subsequent physiological and immunological functions;3.)key physiological features can be identified to scale up the phenotyping of inositol phosphate and myo-inositol metabolism in larger populations.We use laying hens and Japanese quail as the animal models.In the laying hen experiments,we consider the age of the hens,their genetic background,and variations in dietary calcium(Ca)and P concentration as the experimental factors.We investigate phytic acid degradation products and phytase activity in the digestive tract,characterize the hen’s genetic background and the microbiota composition,study absorption and systemic effects of myo-inositol and their interrelationship with different mitochondrial haplotypes,other epithelial functions and smooth muscle contractility,and modulations of the gut-associated and systemic immune system.In the quail studies,samples of intestinal digesta and tissues from birds belonging to a well-defined family structure are investigated for relationships between P utilization and the intestinal microbiota.The data set,together with data obtained in preliminary work,is utilized in comprehensive QTL linkage and association analyses of P utilization,and investigated for functional putative candidate genes.The proposed Research Unit offers the unique opportunity to perform all these studies with the same animals of well-defined genetic background,and integrated bioinformatics analysis of all data.