The process of adding carbohydrates to proteins,called glycosylation,is the most universal and structurally diverse form of posttranslational modification,it being shared by the vast majority of organisms all the way to humans themselves.This process is important for cells in a lot of ways,be it protein stability,secretion or localization.In fungi,glycobiology has been investigated for two main reasons:first,using fungi as model organisms,and second,trying to engineer them into expression systems for recombinant proteins.For the human pathogen Aspergillus fumigatus,an opportunistic,saprophytic fungus that has continuously been gaining importance as a disease agent over the past decades,the possible significance of protein glycosylation for its virulence is another factor that warrants more research..The glycobiology of A.fumigatus has been one focus of research in the lab of Professor Cheng Jin for a lot of years,and major advances have been made identifying key enzymes and characterizing their molecular function.This work aims to further this knowledge,by overexpressing five central glycosylation enzymes in an attempt to functionally characterize these enzymes from a novel point of view.This genetic approach especially allows for an investigation of the possible interplay of different glycosylation components,as several enzymes can be overexpressed simultaneously,thus making a deeper understanding of the complexity of this system possible.Eventually,the results of this work might not only shed a brighter light on the role of protein glycosylation during the A.fumigatus infection process,but they may also provide cues for the improvement of biotechnologically relevant Aspergilli strains.

兰州重离子加速器冷却储存环的成功运行给QED理论检测这个现代物理最核心的研究领域带来重要的机遇。利用兰州重离子加速器冷却储存环，我们进行两个方面的研究：一，相对论能区的高电荷态重离子与原子的碰撞中的x射线角分布、双电子单光子跃迁、以及转移电离过程中关联电子的发射角分布和能谱的测量研究少电子体系中的电子之间的 Breit相互作用；二，通过研究重离子与另一个非常重原子的碰撞过程中准x射线的测量，探测接近QED理论预测的临界库仑场的效应以及通过对重离子的亚稳态寿命的测量来研究QED效应对禁戒跃迁的影响。