泛研网

公众版 查看说明
全球科研项目数据库2025版

酸化等多重环境胁迫对养殖生物生存和生理的影响与生态效应研究

项目来源

国家重点研发计划(NKRD)

项目主持人

刘广绪

项目受资助机构

浙江大学

项目编号

2018YFD0900603

立项年度

2018

立项时间

未公开

研究期限

未知 / 未知

项目级别

国家级

受资助金额

668.00万元

学科

蓝色粮仓科技创新

学科代码

未公开

基金类别

未公开

关键词

酸化 ; 高温 ; 盐度 ; 水产养殖 ; 贝类 ; 甲壳动物 ; 刺参 ; 鱼类 ; acidification ; warming ; salinity ; aquaculture ; mollusk ; Crustacean ; sea cucumber ; teleost

参与者

杨志刚;杨劲树;么宗利;张卫卫

参与机构

上海海洋大学;中国水产科学研究院东海水产研究所;宁波大学

项目标书摘要：为实现阐明多重环境胁迫影响水产物种关键生理过程的分子基础与生态效应这一总体目标，本年度（2019.1-2019.11）根据所研究物种限制性环境因子的不同，综合运用多种技术手段，分析了酸化-高温对贻贝生理代谢、足丝分泌与聚集行为的影响，研究了盐度对中华绒螯蟹性腺发育、生理代谢与渗透压调节的作用，阐释了盐度与甲壳纲模式物种卤虫胚胎休眠与细胞静息间的内在关系，探究了刺参甲基化与甲基转移酶对高温复合低氧的响应，并初步解析了pH-低氧胁迫对多种鱼类代谢的影响。获得的初步结果显示：（1）升温能一定程度上缓解酸化对厚壳贻贝代谢的负面作用；酸化显著削弱了贻贝足丝的分泌与力学性能，并影响了其富集行为；（2）盐度（0-18）升高可加快成蟹性腺发育，调节其机体代谢水平，并影响了鳃Na+/K+-ATP酶活力及其基因表达，从而影响其血清渗透压与离子浓度；（3）通过差异表达谱分析，发现Wnt、AURKA与p53信号通路与卤虫休眠胚胎打破细胞静息密切相关，且此过程伴随着细胞内氯离子的累积与膜电位的显著变化，而Ar-CFTR基因在此过程中发挥着重要的生物功能；（4）在高温低氧胁迫下刺参的生物节律、代谢等通路相关基因甲基化增加，相应地甲基转移酶亦呈现高表达；（5）pH-低氧胁迫能显著影响多种鱼类的代谢。

Application Abstract: In order to elucidating the molecular basis and ecological effects of multiple environmental stressors on the key physiological processes of major aquaculture species, following researches have been carried out this year (January 2019 to November 2019) investigating the impacts of species specific restrictive environmental factors. Through multidiscipline approaches, the project studied the effects of acidification and warming stress on the metabolism, byssus excretion and mechanical characteristics, and aggregation behavior of hard shell mussel; the impacts of salinity on the gonad development, metabolism, and osmotic regulation of Chinese mitten crab; the intrinsic relationship between salinity and embryo dormancy and reactivation of artemia; the methylation of genes and activity of methyltransferase of sea cucumber in response to hypoxia and warming stress; and the effects of pH and hypoxia stress on metabolism of several fish species. The preliminary results obtained showed that: (1) warming could alleviate the negative effect of acidification on metabolism of mussel; acidification significantly weakened the secretion and mechanical properties of byssus, and affected the aggregation behavior of mussels; (2) the increase of salinity (0 to 18) could accelerate the gonadal development of adult Chinese mitten crab, regulate the metabolism level, and affect the activity and gene expression of Sodium Potassium ATPase; (3) the Wnt, AURKA and p53 signaling pathway are crucial for the activation of resting cell of Artemia dormant embryo and this process was accompanied by the accumulation of chloride ions and significant change in membrane potential, during which ArCFTR gene played an important biological function; (4) along with significant upregulation of the expression of methyltransferase, exposure to elevated temperature and reduced oxygen led to significant methylation of genes from circadian and metabolic pathways; (5) the metabolism of several fish species were significantly affected by pH and hypoxia stress.

项目受资助省

浙江省

  • 排序方式：
  • 1
  • /

  • 1.Nanoparticles decrease the byssal attachment strength of the thick shell mussel Mytilus coruscus

    • 关键词：
    • Nanoparticles; Mytilus coruscus; Byssal thread; Mechanical properties;SEAWATER ACIDIFICATION; ENVIRONMENTAL CONCENTRATIONS; ENGINEEREDNANOMATERIALS; BIVALVE MOLLUSKS; QUANTUM DOTS; IMMUNOTOXICITY;RESPONSES; IMPACTS; SIZE; ACCUMULATION
    • Shi, Wei;Guan, Xiaofan;Sun, Shuge;Han, Yu;Du, Xueying;Tang, Yu;Zhou, Weishang;Liu, Guangxu
    • 《25th Topical Meeting of the International-Society-of-Electrochemistry》
    • 2020年
    • MAY 12-15, 2019
    • Univ Castilla La Mancha, Fabrica Armas, Toledo, SPAIN
    • 会议

    The extensive application of nanoparticles (NPs) drives their release into the ocean, which may pose a potential threat to marine organisms. Although the byssus is important for the survival of mussels, the effects of NPs on byssal attachment and the underlying molecular byssal responses remain largely unknown. Therefore, the impacts of three metal oxide NPs (nTiO(2), nZnO, and nFe(2)O(3)) on the production and mechanical properties of byssal thread in the thick shell mussel M. coruscus were investigated in this study. The results showed that both mechanical properties (such as strength and extensibility) and morphology (diameter and volume) of byssal thread newly produced by M. coruscus were significantly affected after NP exposure, which resulted in an approximately 60-66% decrease in mussel byssal attachment strength. Downregulated expression of genes encoding mussel foot proteins, precursor collagen proteins, and proximal thread matrix proteins was also detected in this study, and this alteration may be one of the reasons for the weakened mechanical properties of byssal threads after NP exposure. This study indicated that NP pollution may hamper byssal attachment of M. coruscus and thereby pose a severe threat to the health of mussel individuals and the stability of the intertidal ecosystem. (C) 2020 Elsevier Ltd. All rights reserved.

    ...

  • 2.Nanoparticles decrease the byssal attachment strength of the thick shell mussel Mytilus coruscus

    • 关键词：
    • Nanoparticles; Mytilus coruscus; Byssal thread; Mechanical properties;SEAWATER ACIDIFICATION; ENVIRONMENTAL CONCENTRATIONS; ENGINEEREDNANOMATERIALS; BIVALVE MOLLUSKS; QUANTUM DOTS; IMMUNOTOXICITY;RESPONSES; IMPACTS; SIZE; ACCUMULATION
    • Shi, Wei;Guan, Xiaofan;Sun, Shuge;Han, Yu;Du, Xueying;Tang, Yu;Zhou, Weishang;Liu, Guangxu
    • 《25th Topical Meeting of the International-Society-of-Electrochemistry》
    • 2020年
    • MAY 12-15, 2019
    • Univ Castilla La Mancha, Fabrica Armas, Toledo, SPAIN
    • 会议

    The extensive application of nanoparticles (NPs) drives their release into the ocean, which may pose a potential threat to marine organisms. Although the byssus is important for the survival of mussels, the effects of NPs on byssal attachment and the underlying molecular byssal responses remain largely unknown. Therefore, the impacts of three metal oxide NPs (nTiO(2), nZnO, and nFe(2)O(3)) on the production and mechanical properties of byssal thread in the thick shell mussel M. coruscus were investigated in this study. The results showed that both mechanical properties (such as strength and extensibility) and morphology (diameter and volume) of byssal thread newly produced by M. coruscus were significantly affected after NP exposure, which resulted in an approximately 60-66% decrease in mussel byssal attachment strength. Downregulated expression of genes encoding mussel foot proteins, precursor collagen proteins, and proximal thread matrix proteins was also detected in this study, and this alteration may be one of the reasons for the weakened mechanical properties of byssal threads after NP exposure. This study indicated that NP pollution may hamper byssal attachment of M. coruscus and thereby pose a severe threat to the health of mussel individuals and the stability of the intertidal ecosystem. (C) 2020 Elsevier Ltd. All rights reserved.

    ...
  • 排序方式：
  • 1
  • /