项目来源

国家自然科学基金(NSFC)

项目主持人

马恩波

项目受资助机构

山西大学

立项年度

2001

立项时间

未公开

项目编号

30170612

研究期限

未知 / 未知

项目级别

国家级

受资助金额

15.00万元

学科

生命科学-植物保护学-农业昆虫学

学科代码

C-C14-C1402

基金类别

面上项目

关键词

防治应用 ; 蝗害 ; 群体遗传 ; grasshopper ; allozyme ; molecular markers ; genetic structure of populations ; pesticide resistance ; Pest grasshoppers.Population genetics.Control applicatio

参与者

李春选；郭亚平；黄登宇；任竹梅；郑先云；韩焱

参与机构

湖北大学

项目标书摘要：本项目立论于种群遗传学与分子毒理学的交叉点上，以中国重要农业害虫飞蝗、稻蝗为对象，利用分子水平有效的遗传标记，筛选害虫种群对杀虫剂敏感/耐受的基因型，进而确定杀婕炼曰蛐推德时浠挠跋臁2捎眉际跤械任幻阜治觥FLP、RAPD等。通过上述研究，建⑵鸹瘸嬉糯峁贡浠亩嗖夥椒ǎ员阍诨瘸娣乐沃醒≡衩舾械纳背婕粒岣叻佬А

Application Abstract: Based on the theory of population genetics,this study focus on the genetic stucture of some important grasshoppers in China by using the biochemistry and molecular methods shch as RAPD,AFLP and allozyme techniques.The main results are as follows:(1)The study of population structure in some grasshoppers by using the allozyme techniques and some molecular markers such as RAPD,AFLP.The specimens collected more than ten provinces in China,including Oxya chinensis and Locust migratoria manilensis,the results studied the population genetics in these grasshoppers and showed these genetic markers could be used to reveal the relationships and their genetic structure among the species and the polulations.(2)The study of biochemical mechanisms of pesticide resistance in some pest grasshoppers.The results showed that the increasing insecticide selection pressure was attributed to the decreased insecticide sensitivity in some field populations in Locust migratoria manilensis and Oxya chinensis.Increased general esterases activities were associated with less sensitivity to these pest grasshoppers.(3)On the basis of population genetic structure and divergence of Oxya chinesis,this study examined the relationship of the allozyme genotype and the differential acute mortality of Oxya chinensis.The results suggested that the correlations between the allozyme genotype and toxic effects of pesticides.This correlation upon further confirmations is potentially applicable in monitoring the dynamic changes of allozyme genotypes of the grasshopper species as a pesticide resistance biomarker of Oxya chinenses.We have cooperation research with Kansas State University.The 31 research papers have been published in this research,and 5 Doctors and 3 Masters theses achieved.

项目受资助省

山西省

项目结题报告

中国重要农业蝗虫群体遗传结构及防治应用研究结题报告(全文)