检疫技术之研发与应用
项目来源
台(略)府(略)金(略)B(略)
项目主持人
蔡(略)
项目受资助机构
台(略)府(略)员(略)试(略)物(略)
项目编号
1(略)科(略)4(略)子(略)
立项年度
2(略)
立项时间
未(略)
研究期限
未(略) (略)
项目级别
省(略)
受资助金额
1(略)3(略)千(略)
学科
植(略)
学科代码
未(略)
基金类别
基(略)/(略)究(略)预(略)
关键词
快(略)技(略) (略)物(略)检(略) (略)a(略) (略)g(略)i(略)e(略)i(略)s(略)((略)r(略)i(略)p(略))(略)q(略)n(略)e(略)e(略)e(略)
陈(略)谢(略)邱(略)陈(略)杨(略)林(略)路(略)萧(略)苏(略)阮(略)林(略)邓(略)邱(略)李(略)黄(略)叶(略)郑(略)陈(略)李(略)陈(略)颜(略)张(略)何(略)颜(略)郭(略)李(略)柯(略)许(略)王(略)林(略)张(略)朱(略)
参与机构
未(略)
项目标书摘要:本子(略),分述如下一、植物(略)、检疫处理技术与程(略)一)边境查验检出检(略)分析系统本专家系统(略)进行样品监定分析,(略)之病、虫、蟎等有害(略)处理之依据。本(1(略)期效益包括,1.强(略)作业分层负责系统((略)害生物监定系统之功(略)物细菌及鞘翅目害虫(略)层检疫人员监定能力(略)急件定样品之正确性(略)品监定规划与处理,(略)统之功能提升检疫监(略)物病原性叶芽线虫快(略)口栽培介质及植株附(略)植物检疫线虫有比例(略)有效预防高风险性外(略)叶芽线虫属内种间之(略),建立属内重要检疫(略),并开发用於监定检(略),以供未来检疫人员(略)用。(三)建立检疫(略)术、疫情资讯及教育(略)果实蝇物种诊断监定(略)地区检防疫果实蝇名(略)要果实蝇害虫疫情资(略)监定能力之教育训练(略)商谈判,常涉及国内(略)否的问题,为避免谈(略)优先厘清国内外果实(略)实蝇之形态监定图谱(略)人员使用,以争取时(略)蜜枣冷藏检疫处理技(略)日之限制,拓展蜜枣(略)95年间即成功开发(略)经日方派员前来认证(略)术。经认证结果,确(略)果心中心温度1.2(略),於105年开放我(略)然近几年国产蜜枣品(略)广泛栽植品种,开发(略)冷藏处理杀虫条件,(略)减少冷藏处理所需费(略))我国重要检疫病毒(略)析及检测技术开发为(略)病毒类资料之准确性(略)病毒清单中100笔(略)增列重要植物检疫病(略),我们也将蒐集感染(略),选择高入侵风险的(略)料,设计病毒的专一(略)基础的广效性引子对(略)R测试方法的准确性(略)物清单,以及所研发(略),提供给植物检疫单(略)输入植物清单与检疫(略)处理维护「核准输入(略)料库与正面表列条文(略)见进行条文内容处理(略)处理规则并将其补充(略)检疫条件」资料库,(略)升条文资料覆盖率。(略)线虫与食菌性茎线虫(略)收集数个茎线虫种类(略)所有线虫所具之一般(略)集之茎线虫DNA。(略)线虫核酸序列以产生(略)将测试探针专一性,(略)境检疫来区别检疫重(略)茎线虫种类。台湾地(略)因为高海拔地区也有(略)由温带地区输入有害(略)险也不小。边境检疫(略)道防线,建立一个省(略)可以节省许多人力以(略)进出口业务处理速度(略)整合性防治技术开发(略)兰、蕙兰与嘉德丽雅(略)口竞争压力中,如何(略)率,是增加竞争优势(略)花在栽培过程中,病(略)下降的主要原因之一(略)发生,研拟可行的病(略)对症下药,但也常导(略)虫害发生时,因误诊(略)态,非但无法对症下(略)发生的最佳时机。另(略)的发生,特别是害虫(略)碍与出货品质不佳。(略)兰与嘉德丽雅兰常见(略)、园区病虫害管理作(略)作法,建立病虫害的(略)田间实际推广使用。(略)检测监定技术之研发(略)台湾分布探究及其与(略)计划拟调查及采集国(略)类,并与国外研究单(略)行快速监定技术开发(略)、快速的目标,并厘(略)分布危害。计划目标(略)莓及葡萄园分布的果(略)防疫害虫斑翅果蝇之(略)。3.利用生物技术(略)见果蝇标本监定技术(略)疫。(二)开发农业(略)萤金花虫金花虫科((略)ae)为为鞘翅目((略)中种类最多样的一个(略)过六百种;往昔的研(略)录的发表,且采集地(略)代化分类学研究的开(略)yrrhalta (略)定期性的方式采集标(略),详细研究每个种类(略)期、寄主植物及地理(略)的模式标本,以建立(略)断监定研究。(三)(略)加值应用之研究盘点(略)清及抗原种类,去芜(略)发制作瓜类及葱蒜病(略)检测费用;测试比较(略)列出各自的优缺点,(略)测试剂组之商品化及(略)测技术开发与应用建(略)-PCR检测系统,(略)进口检测工具。并利(略)在茄科作物发生情形(略)子带毒率与传播特性(略)薇科果树检测系统,(略)树发生现况。以接种(略)厘清种子与土壤传播(略)依据。(五)进口百(略)剂整合性开发及应用(略)包括百合、郁金香、(略)其病毒发生种类资料(略),以强化百合科花卉(略)效能。建立百合科进(略)化防检疫监测能力。(略)病害之监定与防治番(略),且为连续采收作物(略)中於台湾田间可造成(略)青枯病菌(Rals(略)acearum)、(略)cterium c(略)subsp.car(略)年新发现髓坏疽病((略) viridifl(略)rii、P.med(略)萎凋型病害常造成番(略)防治药剂可推荐。本(略)立一快速的诊断流程(略)各病菌PCR分析,(略)断所感染的病菌,并(略)的拮抗微生物及防治(略)菌性病害。五、马铃(略)对技术建立本研发收(略)病毒),并建档於分(略)马铃薯材料抽取总量(略)NA解序,每材料解(略)tig及blast(略)料库,列出可能性((略)-PCR或ELIS(略)次世代解序技术,开(略)检测技术,并开发分(略)料的病原检定。
Applicati(略): This pr(略)ded 5 det(略) fellow.A(略)pment,imp(略)d applica(略)k analysi(略)e technol(略)cedures f(略)nd export(略) plant pr(略)egrated s(略)dentifica(略)alysis of(略) pests in(略)y border (略)The goal (略)grated sy(略) invites (略)tes domes(略)entificat(略) to provi(略)ic suppor(略)dentifica(略)nt pests (略)lant dise(略)s,mites a(略)ests on i(略) exported(略)al produc(略) of resea(略)and expec(略)s of this(略)de 1)To s(略)he expert(略)tion syst(略)n the fun(略)mestic qu(略)entificat(略)s of plan(略),insects (略))To set u(略)ification(略)ourse of (略)d beetles(略)arantine (略) each bra(略)to enhanc(略)ty of qua(略)ntificati(略) and assu(略)ty of dom(略)ultural p(略)o systemi(略) procedur(略)ergency e(略)rantine p(略)dentifica(略)ance the (略) the effi(略)uarantine(略)ification(略)m will fa(略)e complet(略)domestic (略)identific(略)m and hom(略) with the(略)nal syste(略)shment of(略)tion assa(略)rtant pla(略)c nematod(略)enchoides(略) the incr(略)ction of (略)lant para(略)odes in t(略)d importi(略) material(略)e plantin(略) attached(略)orting pl(略)portance (略) effectiv(略)ient insp(略)identific(略)ds at qua(略)e been el(略)revent th(略)of high r(略)ine nemat(略)he goal o(略)ect is to(略)xisting d(略)ssays and(略)itable pr(略)the detec(略) importan(略) in the A(略)es genus.(略)t first e(略)e biologi(略),the mole(略)mation an(略)ing DNA b(略)lar detec(略) targetin(略)ntine nem(略)then deve(略)ve and ef(略)ecular as(略)timize th(略)lable ass(略)our quara(略)ction nee(略)s project(略) DNA bioc(略)ng quaran(略)tion tool(略)identific(略)e importa(略)ne nemato(略)lishment (略)ic techni(略)c informa(略)ucation t(略)tems of f(略)Diptera:T(略)with quar(略)rtanceThi(略)ims to es(略)ore conci(略)rate frui(略)mation an(略)s databas(略) 1)Revisi(略)y species(略)in Taiwan(略)ng intern(略)it fly ep(略);3)Streng(略)it fly di(略)lity and (略)est speci(略)ion.Confi(略) fly spec(略)antine im(略) usually (略)t issue i(略)ral trade(略)ns.To pre(略)ssary dif(略)ing quara(略)ction and(略)tiations,(略)the speci(略)ic atlas (略),distribu(略)st inform(略)be useful(略)or the qu(略)d trade n(略)purpose,b(略) educatio(略)cultural (略)s.4.Cold (略)treatment(略)fruit for(略)japan In (略)eak throu(略)rictions (略) jujube f(略)pand the (略)et,Taichu(略)uccessful(略)d the exp(略)ine treat(略) for juju(略) the 2006(略)se govern(略)fficer co(略)n which c(略)n of the (略)treatment(略) for the (略)ding to t(略)ation res(略)confirmed(略)uarantine(略) for the (略)efrigerat(略)t at the (略)he fruit (略) °C for 1(略)he year o(略)fruit of (略) dates wa(略) Japan.Ho(略)cent year(略)ties of d(略)es have b(略)ously imp(略) planned (略)the insec(略)ditions o(略)ting trea(略)temperatu(略)C for 12 (略)the curre(略)ultivated(略)and reduc(略)gerating (略)ime,in or(略)ce the co(略)gerating (略)nd increa(略)income.5.(略)al outbre(略) and deve(略)detection(略)r importa(略)ne viruse(略)to mainta(略)racy of o(略)ne pest l(略) continue(略)ing and c(略)he classi(略)d scienti(略) 100 list(略)and also (略)ortant qu(略)ruses to (略)st.In add(略)ll collec(略)mation of(略)mitted pe(略)s and sel(略)uses with(略)of invasi(略)ts.Virus-(略)imers or (略)fic broad(略)rimers wi(略)ned based(略)equence d(略)ection ac(略) be teste(略)ep RT-PCR(略)ecessary,(略)d phytosa(略)s dried m(略)l be purc(略)foreign r(略)titutions(略)sed to te(略)ificity o(略)r pairs.F(略)revised q(略)irus list(略)tection m(略)eed-trans(略)er viruse(略)rovided t(略)rities of(略)ection an(略)e.6.The M(略)and Data (略)of Search(略)Approved (略)for Impor(略)its Quara(略)rements T(略) the data(略)rantine R(略) for the (略) of Plant(略)Products (略)public of(略)ibited im(略)ts”:The m(略)include d(略)ng,specia(略)e rules p(略)ata impor(略)stem test(略)ctions pr(略)he databa(略)informati(略)l,data ma(略)d user ma(略) maintain(略)bases“App(略)ted plant(略)Quarantin(略)nts for t(略)ion of Pl(略)nt Produc(略) Republic(略)Including(略)ement,and(略) will be (略)cording t(略)ack of us(略) optimiza(略) page int(略)ntegrate (略)es“Approv(略) plants l(略)ses“Quara(略)rements f(略)rtation o(略) Plant Pr(略) the Repu(略)na”,and“Q(略)equiremen(略)Importati(略)s or Plan(略)into the (略) China-Pr(略)ported pl(略) platform(略)earch Sys(略)oved Plan(略)Importati(略)Quarantin(略)nts”.To c(略) training(略)se promot(略)oping mol(略)es to dis(略)arantine (略)feeding D(略)speiciesS(略)lenchus s(略)ons as we(略)is DNA wo(略)ected for(略) the spec(略)c probes.(略)quarantin(略) Ditylenc(略) would be(略)from othe(略)he foreig(略) to provi(略)base for (略)Conserve (略)h as ITS (略)ions of t(略)s would b(略) to locat(略)tial posi(略)veloping (略)-specific(略)en the pr(略)signed,se(略)enchus sp(略)ns includ(略)that only(略)ngal hyph(略) included(略)e sensibi(略) primers.(略)ted at th(略)and subtr(略),but the (略)tude moun(略)s is suit(略)sts from (略)egions.Ha(略)olecular (略)etecting (略)Ditylench(略)correctly(略)nt,since (略)of nemato(略) as the s(略) not be a(略)ide enoug(略)c informa(略)ping the (略)specific (略)ld help s(略) quaranti(略)ion proce(略) time as (略)ors.C.Dev(略) integrat(略)technique(略)ant disea(略)ts for ex(略)ids Taiwa(略)export or(略)halaenops(略)m and Cat(略)o on.Thos(略)ave expor(略) from int(略)competiti(略)ncement o(略) quality (略)he feasib(略)increase (略) advantag(略)uring the(略)n of orch(略)urrence o(略) diseases(略)the main (略) the decl(略)d and qua(略) past,the(略)t of a vi(略)anagement(略)as follow(略)c single (略)pest.But,(略)rs may mi(略) or don't(略) the occu(略)est and d(略)ogy when (略)with diff(略)ses and p(略)ot only f(略)medy the (略) also del(略)st time t(略)ests.In a(略) occurren(略) and dise(略)ally the (略) pests,of(略)o export (略)d poor qu(略)livery at(略)f export (略)Focusing (略)pective o(略)dling sys(略)t managem(略)greenhous(略)y will ai(略)pment an (略)pest mana(略)ommon pes(略)ases of P(略),Cymbidiu(略)eya.D.Dev(略)d applica(略) techniqu(略)nosis and(略)tion of i(略)op diseas(略)velopment(略)iagnosis (略)and distr(略)vey of sp(略)drosophil(略)a suzukii(略)This proj(略) survey a(略)fruit fly(略) strawber(略) and vine(略)n specime(略)phila suz(略)oreign re(略)itutes.Us(略)cimens fo(略)ntificati(略)ical deve(略) make ide(略) easier a(略)t also cl(略)ther ther(略)ribution (略)la suzuki(略).The proj(略) focus on(略)ing items(略)ation of (略)species d(略)in strawb(略)ds and vi(略)stablish (略)ntificati(略)e and sta(略)tion proc(略)or import(略)rosophila(略)otted win(略)a).3.Esta(略)id and si(略)fication (略)or common(略) specimen(略) to enabl(略) applicat(略) quaranti(略)fication (略)ct pests (略) genus Py(略)ysomelida(略) the most(略)milies of(略).Morethan(略)d species(略)described(略)d from Ta(略)r,most of(略)studies o(略)ids were (略)toshort d(略) of new s(略)nnounceme(略)ecords.Th(略) sites of(略)d specime(略)ays scatt(略)enumber o(略)ed specim(略)wer as ex(略) research(略)d to the (略)nomic stu(略)ysomelida(略).The spec(略)ng to the(略)halta are(略)prehensiv(略)ns will b(略) intensiv(略)nsively.D(略)haracters(略)arched th(略)lmembers (略)s.Adult a(略)ost plant(略)eographic(略)n of each(略)ll be maj(略)ts of thi(略)The type (略) the know(略)ill be re(略)soundclas(略)of Chryso(略)Taiwan is(略)o be esta(略)esearch o(略)ed applic(略)ventory p(略)ntisera t(略)uses Make(略)ry of ant(略)ruses of (略)nd allium(略)and to ke(略) collecti(略)ning test(略)s to deve(略)le antise(略) to produ(略)n kits th(略)uce the c(略)utine tes(略)ison of s(略)agents an(略)l product(略)r strengt(略)nesses,an(略)e the aut(略)of techno(略)er or com(略)ion of de(略)s.4.Devel(略) applicat(略)ction tec(略) viroid R(略)scriptase(略)e establi(略)ect viroi(略)ers was d(略)ording to(略)equences (略)The viroi(略)tomato wi(略)yed first(略)xtended t(略)d fruit.T(略)infected (略)aining Ch(略) stunt vi(略)unt viroi(略)o chlorot(略)roid will(略)d by this(略)PCR detec(略) develope(略)tudy,can (略)seed impo(略)rt inspec(略)The syste(略)sed to fi(略)gation to(略)e type of(略)ected Sol(略)ps,and ho(略) the viro(略)sults can(略) basis fo(略)anagement(略)ent and a(略)of viral (略)eagents f(略)rted lili(略)ropsThe o(略) this stu(略)velop the(略)tabase an(略) reagents(略)amental b(略)long to t(略)e,and to (略) efficien(略) diagnosi(略)ported li(略)bs.To dev(略)rus-index(略) to inten(略)arantine (略)ion of im(略)aceae bul(略)ping iden(略)and contr(略)es for th(略) wilt typ(略)f tomato (略) is one o(略)tant vege(略)iwan.Many(略)disesses (略)ould be f(略) field.Th(略) pathogen(略)alstonia (略)m,Pectoba(略)otovorum (略)ovorum,Ps(略)iridiflav(略)i and P.m(略).These ba(略)d result (略)ptom on t(略)se there (略)gested ag(略) to contr(略)sease.For(略)d diagnos(略)hogen,PCR(略)and bioco(略) will be (略) for rapi(略)ing and m(略)hese dise(略)establish(略)h through(略)cing tech(略)tect pota(略) virus-in(略)to materi(略) confirme(略)gen of ri(略)tradition(略) ELISA,RT(略)extractin(略) amount o(略)2 virus-i(略)ato mater(略)all-molec(略)ld be ext(略)sequenced(略)ount sequ(略) be 5 Meg(略)ng contig(略) software(略)generated(略) RNA 20-3(略)e compare(略)e with th(略) pathogen(略)nd the po(略)noculated(略)ill be li(略)tudy uses(略)eneration(略)g technol(略)lop a det(略)nique for(略)ic viral (略)nd develo(略)tical pro(略)an be app(略) pathogen(略)ntroduced(略)
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