项目来源

国家自然科学基金(NSFC)

项目主持人

袁肇凯

项目受资助机构

湖南中医药大学

项目编号

30772696

立项年度

2007

立项时间

未公开

项目级别

国家级

研究期限

未知 / 未知

受资助金额

29.00万元

学科

医学科学-中医学-中医诊断学

学科代码

H-H31-H3106

基金类别

面上项目

关键词

干细胞移植 ; 机理 ; 骨髓间充质干细胞 ; 心肌缺血 ; 养心通脉有效部位方 ; myocardial ischemia ; bone marrow mesenchymal stem cells ; stem cell transplantation ; active principle region of Yangxing Tongmai Formula(apr-YTF)

参与者

黄献平；胡志希；谢梦洲；毛以林；孙贵香；简维雄；莫莉；陈清华

参与机构

云南中医药大学

项目标书摘要：心肌缺血性疾病一直是临床治疗的难点，通过骨髓间充质干细胞(MSCs)移植以改善缺血心肌功能，重建梗死心肌丧失的心肌细胞虽已显示出良好的应用前景，但目前还存在着①MSCs体外诱导分化为心肌细胞的转化率较低,②MSCs移植于梗死心肌的存活率较低,③骨髓干细胞动员归巢于心肌的百分率较低等一些亟待解决的问题，而制约了缺血心肌干细胞移植的临床应用。养心通脉有效部位方是中医治疗胸痹心痛的有效名方，针对上述MSCs心肌移植的3个问题，本项目通过养心通脉有效部位方诱导大鼠骨髓间充质干细胞体外心肌样细胞分化的研究，对骨髓间充质干细胞移植大鼠梗死心肌存活影响的研究，及动员骨髓干细胞归巢大鼠梗死心肌特性变化的实验研究，以探讨养心通脉有效部位方对骨髓间充质干细胞在移植梗死心肌的过程中"诱导分化"、"移植存活"、"动员归巢"等三个环节的影响，以阐明养心通脉有效部位方调控缺血心肌骨髓干细胞移植的作用靶点及其机理。

Application Abstract: o observe the effect of active principle region of Yangxing Tongmai Formula(apr-YTF)on mesenchymal stem cells(BMSCS)of ischemic myocardial transplantation The study contains the following.① The reserch of apr-YTF induced MSCs differentiated to cardiomyocyte-like cells in vitro:myocardial structural protein Desmin,muscle and cardiac myosin heavy chain MHC restricted promoter factor GATA-4 mRNA in apr-YTF group were significantly higher than the healthy group and the positive control group(5-aza group)(P<0.05);② The effect of the number of survival BMSCs on transplant of myocardial infarction in rats treated by apr-YTF:of Connexin 43(CX43)and the early development of the heart NKX2.5 gene in[apr-YTF]+BMSCs group was significantly higher than in the healthy group and the positive control group([rhG-CSF]+BMSCs group)(P<0.05);③The reserch of apr-YTF mobilization BMSCs homing to rat infarcted myocardium:The cells of CD34+and Brdu and cTn I double staining positive in apr-YTF group,markers of BMSCs in cardiac tissue was significantly higher than model group and rhG-CSF group(P<0.01).④The effect of heart blood stasis syndrom“microenvironment”on the is MSCs transplantation:Matrix metalloproteinase-2,matrix metalloproteinase-9,(MMP-2,MMP-9)higher expression and signal transduction pathway PI3K,p38-related protein changed in myocardial ischemia related with BMSCS transplantated.Conclusion:apr-YTF regulation BMSCs transplantation into ischemic myocardium by the way“differentiation”,“transplantation survival”and“mobilization”.

项目受资助省

湖南省

项目结题报告

养心通脉有效部位方对大鼠缺血心肌骨髓间充质干细胞移植的影响结题报告(全文)