Weill Cornell/Rockefeller/Sloan Kettering MST Program
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1.Hyperpolarized [6-C-13,N-15(3)]-Arginine as a Probe for in Vivo Arginase Activity
- 关键词:
- MAGNETIC-RESONANCE; CANCER METABOLISM; L-ARGININE; BIOMARKERS; CELLS
- Cho, Andrew;Eskandari, Roozbeh;Granlund, Kristin L.;Keshari, Kayvan R.
- 《7th Annual Conference of the International-Chemical-Biology-Society - Toward Translational Impact》
- 2019年
- SEP 24-27, 2018
- Vancouver, CANADA
- 会议
Alterations in arginase enzyme expression are linked with various diseases and have been shown to support disease progression, thus motivating the development of an imaging probe for this enzymatic target. C-13-enriched arginine can be used as a hyperpolarized (HP) magnetic resonance (MR) probe for arginase flux since the arginine carbon-6 resonance (157 ppm) is converted to urea (163 ppm) following arginase-catalyzed hydrolysis. However, scalar relaxation from adjacent N-14-nuclei shortens cabon-6 T-1 and T-2 times, yielding poor spectral properties. To address these limitations, we report the synthesis of [6-C-13,N-15(3)]-arginine and demonstrate that N-15-enrichment increases carbon-6 relaxation times, thereby improving signal-to-noise ratio and spectral resolution. By overcoming these limitations with this novel isotope-labeling scheme, we were able to perform in vitro and in vivo arginase activity measurements with HP MR We present HP [6-C-13,N-15(3)]-arginine as a noninvasive arginase imaging agent for preclinical studies, with the potential for future clinical diagnostic use.
...2.Stimulation of Hair Cells with Ultraviolet Light
- 关键词:
- MECHANOELECTRICAL-TRANSDUCTION; ADAPTATION; AMPLIFICATION; CHANNELS;MODEL; CA2+
- Azimzadeh, Julien B.;Fabella, Brian A.;Hudspeth, A. J.
- 《13th Mechanics of Hearing Workshop on To Ear and Back Again -Advances in Auditory Biophysics》
- 2018年
- JUN 19-24, 2017
- St Catharines, CANADA
- 会议
Hair bundles are specialized organelles that transduce mechanical inputs into electrical outputs. To activate hair cells, physiologists have resorted to mechanical methods of hair-bundle stimulation. Here we describe a new method of hair-bundle stimulation, irradiation with ultraviolet light. A hair bundle illuminated by ultraviolet light rapidly moves towards its tall edge, a motion typically associated with excitatory stimulation. The motion disappears upon tip-link rupture and is associated with the opening of mechanotransduction channels. Hair bundles can be induced to move sinusoidally with oscillatory modulation of the stimulation power. We discuss the implications of ultraviolet stimulation as a novel hair-bundle stimulus.
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