项目来源

巴西圣保罗研究基金(FAPESP)

项目主持人

Matheus Bertanha

项目受资助机构

Faculdade de Medicina.Universidade Estadual Paulista.Campus de Botucatu.Botucatu,SP,Brazil

项目编号

21/10258-1

立项年度

2021

立项时间

未公开

研究期限

未知 / 未知

项目级别

国家级

受资助金额

未知

学科

Health Sciences

学科代码

未公开

基金类别

Scholarships in Brazil-Scientific Initiation

关键词

Técnicas histológicas ; Procedimentos cirúrgicos vasculares ; Cirurgia ; Engenharia tecidual ; Vasos sanguíneos ; Matriz extracelular ; Veias ; Tecidos suporte ;

参与者

Eduarda Martins Garcia

参与机构

未公开

项目标书摘要：It is well established that,as a form of treatment for the peripheral arterial occlusive disease in a situation of critical ischemia,patients can undergo endovascular techniques and/or bypass surgery.In conventional surgeries,an ideal vascular substitute would be a blood vessel with the same characteristics as the artery to be replaced.However,this therapy cannot always be used for all patients and the treatment alternative ends up being the use of synthetic materials whose patency it's smaller.Tissue engineering emerges as a promising proposal as an alternative for providing personalized tissues and organs,aiming to produce biocompatible grafts to replace damaged or non-functional organs or tissues.Objective:To perform the decellularization of human saphenous veins to produce a 3D scaffold for future use in tissue engineering in blood vessels,comparing sufficient and insufficient saphenous veins regarding the decellularization process.Methods:morphofunctional characterization of decellularized great saphenous veins will be performed,through the test of two decellularizing agents(SDS and DS)in two concentrations(2%and 3%)maintained for 1 and 2 hours under continuous agitation.Sufficient human great saphenous veins will be used from 7 individuals undergoing surgical procedures to make bypass/bypass with sufficient great saphenous veins and that have an unused residual segment(in natura),in addition to insufficient great saphenous veins from 8 individuals undergoing removal surgical procedure for the treatment of chronic venous insufficiency.The frameworks will be comparatively analyzed for the best decellularization process by histological analysis of the extracellular matrix.Results:It is hoped that we can obtain sufficient information to choose the best method of decellularization of sufficient and insufficient great saphenous veins for use in blood vessel tissue engineering.(AU)

Application Abstract: It is well established that,as a form of treatment for the peripheral arterial occlusive disease in a situation of critical ischemia,patients can undergo endovascular techniques and/or bypass surgery.In conventional surgeries,an ideal vascular substitute would be a blood vessel with the same characteristics as the artery to be replaced.However,this therapy cannot always be used for all patients and the treatment alternative ends up being the use of synthetic materials whose patency it's smaller.Tissue engineering emerges as a promising proposal as an alternative for providing personalized tissues and organs,aiming to produce biocompatible grafts to replace damaged or non-functional organs or tissues.Objective:To perform the decellularization of human saphenous veins to produce a 3D scaffold for future use in tissue engineering in blood vessels,comparing sufficient and insufficient saphenous veins regarding the decellularization process.Methods:morphofunctional characterization of decellularized great saphenous veins will be performed,through the test of two decellularizing agents(SDS and DS)in two concentrations(2%and 3%)maintained for 1 and 2 hours under continuous agitation.Sufficient human great saphenous veins will be used from 7 individuals undergoing surgical procedures to make bypass/bypass with sufficient great saphenous veins and that have an unused residual segment(in natura),in addition to insufficient great saphenous veins from 8 individuals undergoing removal surgical procedure for the treatment of chronic venous insufficiency.The frameworks will be comparatively analyzed for the best decellularization process by histological analysis of the extracellular matrix.Results:It is hoped that we can obtain sufficient information to choose the best method of decellularization of sufficient and insufficient great saphenous veins for use in blood vessel tissue engineering.(AU)