项目来源
国家重点研发计划(NKRD)
项目主持人
杨华勇
项目受资助机构
浙江大学
项目编号
2018YFA0703000
立项年度
2018
立项时间
未公开
项目级别
国家级
研究期限
未知 / 未知
受资助金额
2735.00万元
学科
变革性技术关键科学问题
学科代码
未公开
基金类别
未公开
关键词
生物制造 ; 功能化组织/器官 ; 评价体系 ; Biofabrication ; Functional Tissues/Organs ; Evaluation system
参与者
马梁；张斌；李琦
参与机构
未公开
项目标书摘要：活性组织/器官制造有着巨大的临床需求，是先进制造技术的前沿发展方向。目前，活性组织/器官体外精确制造仍面临诸多挑战:其一、组织/器官包含多尺度复杂异质结构，如何在数学上描述这些异质结构，并根据不同组织类型需求确定最优的材料及制造方法是一个挑战。其二、生物墨水作为一种典型的软材料，其打印过程中的流变、交联、融合、坍塌都严重影响载细胞结构的最终制造精度，使得其精准成形也是一个挑战。其三、组织发育形成功能的过程中，细胞外基质及各种物理化学载荷对细胞的活性及功能形成至关重要，因而如何更好的模拟细胞外基质及组织发育所受到的物理化学载荷环境，从而实现体外活性结构向功能组织转化也是一个挑战。针对上述挑战，本项目拟系统解决组织/器官体外精准制造的三大科学问题:“体外生物组织/器官宏—微结构设计与优化理论”、“生物墨水打印流变与交联耦合成形机理”、“多场耦合诱导下打印结构向功能化组织转变机制”,攻克从材料研发(微环境可控生物墨水)到精准成形工艺设计(多细胞精确排布及预血管化)到装备(多工艺融合多材料超精密生物打印机)开发等系列关键技术，建立体外组织/器官的物理及生物功能评价体系，为组织/器官的体外精准制造提供系统支撑。
Application Abstract: It is a huge clinical needs for active tissue/organ manufacturing which is the cutting-edge development direction of advanced manufacturing technology.At present,the precise manufacturing of active tissues/organs in vitro still faces many challenges:First,tissues/organs contain multi-scale complex heterogeneous structures.How to mathematically describe these heterogeneous structures and determine the optimal materials and manufacturing methods according to the needs of different tissue types is a challenge.Second,as a typical soft material,the rheology,cross-linking,fusion,and collapse during the printing process seriously affect the final manufacturing accuracy of the cell-containing structure,making its precise formation a challenge.Third,during the process of tissue development and formation,the extracellular matrix and various physical and chemical loads are important to the cell's activity and function formation.Therefore,how to better simulate the physical and chemical load environment to which the extracellular matrix and tissue development are subjected.Therefore,it is also a challenge to realize the transformation of in vitro active structure into functional tissue.In response to the above challenges,this project intends to systematically solve the three major scientific problems of precise manufacturing of tissues/organs in vitro:"External biological tissue/organ macro-microstructure design and optimization theory","Coupling mechanism of rheology and cross-linking of bio-ink printing""The mechanism of printing structure transition to functional organization under the guidance of multi-field coupling",conquering from material development(microenvironment controllable bio-ink)to precision forming process design(multi-cell precise arrangement and pre-vascularization)to equipment(multi-process Integrate a series of key technologies such as multi-material ultra-precision bioprinter)development,establish an evaluation system for the physical and biological functions of tissues/organs in vitro,and provide systematic support for the precise manufacturing of tissues/organs in vitro.
项目受资助省
浙江省
