Application Abstract: The objective of the present study is to evaluate the relationship of semen quality and fertility of boars in artificial insemination(AI)station.The traits of semen quality are evaluated by microscopy and flow cytometry.We will establish an effective method and select some traits highly corrected with boar fertility to monitor the quality of boar semen.The loss of low reproductive efficiency of sows will decrease because of culling the boars with poor semen quality and low fertility in AI stations.First,we will establish flow cytometric assays including mitochondrial function and viability of sperm.The sperm motility and morphology of semen samples are also estimated by phase-microscopy.Some semen samples are inseminated to sows to estimate the fertility of boars.Finally,we will analysis the correlation of boar fertility with semen traits(motility,morphology,viability and mitochondrial function)examined by flow cytometry and microscopy.We hope to establish valuable assays to evaluate the semen quality and monitor the boar fertility in breeding farms and AI stations.They pay much attention to the quality control of boar semen and decrease the number of sub-fertile boars.The reproductive efficiency of boars in breeding farms and AI stations will be improved.
