BACKGROUND: We examined whether total and regional fat-to-muscle ratio (FMR) predicts incident metabolic dysfunction-associated steatotic liver disease (MASLD) and other chronic liver outcomes.; METHODS: Among 356,833 UK Biobank participants (men: 168,784 [47.3%]; women: 188,049 [52.7%]; mean age 56.3years), body composition was assessed by segmental bioelectrical impedance analysis (BIA), from which whole-body and regional (arm, leg, trunk) FMRs were derived and categorized into sex-specific tertiles. Tertile cutpoints (lower and upper) were: men-whole-body (0.309, 0.396), arm (0.264, 0.333), leg (0.254, 0.325), trunk (0.348, 0.453); women-whole-body (0.526, 0.679), arm (0.500, 0.692), leg (0.640, 0.778), trunk (0.462, 0.621). Cox proportional hazards models were applied. MRI subsets included liver proton density fat fraction (PDFF) (n=32,191) and hepatic fibro-inflammation (iron-corrected T1 [cT1]) (n=27,028).; RESULTS: Over a median follow-up of 14.0years, there were 4,404 MASLD, 1,392 cirrhosis, 947 liver-related mortality, and 147 HCC events. Comparing the highest (T3) versus the lowest (T1) tertile of FMR, MASLD risk increased in both sexes across whole-body and regional FMRs (HR 2.29-2.76), with the strongest associations for leg FMR in women (HR 2.95). For cirrhosis and liver-related mortality, T3 associations were mainly seen in men and were strongest for whole-body and trunk/arm FMR (HR 1.63-2.11), while women showed weak/null associations (HR 1.29-1.54). For HCC, T3 FMR was strongly associated in men (HR 3.03-4.71), while no clear associations were evident in women.; CONCLUSION: Higher total and regional FMR predicts MASLD and advanced liver outcomes with clear sex- and region-specific patterns. © 2026. The Author(s), under exclusive licence to Asian Pacific Association for the Study of the Liver.

BACKGROUND: The association of plasma metals on the risk of cardiovascular diseases (CVD) in adults with prediabetes remains poorly investigated. To assess the association between plasma metal exposure and the risk of CVD in prediabetic adults in the United States using five plasma metals.; METHODS: Five cycles of data (2011-2012, 2013-2014, 2015-2016, and 2017-2018) from the NHANES were adopted in this study. The plasma metals were measured in 1088 participants with prediabetes. We utilized multivariate logistic regression, WQS, and BKMR models to evaluate the associations between the five plasma metals and the risk of CVD.; RESULTS: The risk of CVD in participants with prediabetes were found to link to the 2nd quartile, 3rd and 4th quartiles of cadmium on the basis of multivariate logistic model (OR=3.03, 95%CI: 1.17-7.82, P<0.01). Moreover, the joint effect of the five metals on the risk of CVD participants with prediabetes were unveiled using WQS and BKMR models (OR=1.79, 95%CI: 1.15-2.77, P<0.01). In addition, when the concentrations of the other four metals were controlled at the 25th, 50th, and 75th percentile, correspondingly, cadmium had a statistically significant positive association with the risk of CVD.; CONCLUSION: The exposure of metals documented by the five metals links to the risk of CVD in participants with prediabetes in the United States. Among all the five metals, cadmium has the strongest association with the risk of CVD in participants with prediabetes. © 2025. The Author(s).

Objective: Little has been known on the effect of chronic glyphosate exposure on osteoarthritis (OA). The aim of this study was to investigate the association between glyphosate exposure and OA and to further investigate the different moderating effects of leisure time physical activity (LTPA) and body mass index (BMI) types on the association between glyphosate exposure and OA. Methods: Cross-sectional data from 2540 participants in the 2015–2018 National Health and Nutrition Examination Survey (NHANES) were used to explore the association between glyphosate exposure and OA. Multivariate logistic regression models and restricted cubic spline models were used to investigate the association between glyphosate exposure and OA, and further analyses were conducted to determine the association between glyphosate exposure and OA under different LTPA and BMI types. Results: Of the 2540 participants, 346 had OA. Participants with the highest glyphosate concentration (Q4) had a higher incidence of OA compared to participants with the lowest glyphosate concentration (Q1) (OR, 1.88; 95 % confidence interval [CI]: 1.13, 3.13), there was no nonlinear association between glyphosate and OA (non-linear P = 0.343). In the no LTPA group, glyphosate concentration in the Q4 group was correlated with OA (OR, 2.65; 95%CI: 1.27, 5.51). In the obese group, glyphosate concentration in the Q4 group was correlated with OA (OR, 2.74; 95 % CI: 1.48, 5.07). Among people with high BMI and inactive in LTPA, glyphosate concentrations in Q4 were associated with OA (OR, 2.19; 95 % CI: 1.07, 4.48). Conclusions: Glyphosate is associated with OA odd, and physical activity and moderate weight loss can mitigate this association to some degree. This study provides a scientific basis for rational prevention of OA by regulation of LTPA and BMI under glyphosate exposure. © 2024 Elsevier B.V.

BACKGROUND: Live dietary microbes have been hypothesized to promoting human health. However, there has been lacking perceptions to crystallize nexus between consumption of foods with live microbes and mortality.; OBJECTIVE: To investigate the association of consumption of foods with medium to high amounts of live microbes with all-cause, cancer-specific, and cardiovascular disease (CVD)-specific mortality.; METHODS: The data were obtained from the National Health and Nutrition Examination Survey 1999-2018 at baseline linked to the 2019 National Death Index records. Based on consumption of foods that were categorized as either having medium or high microbial content (MedHi foods), participants were classified into three groups. Kaplan-Meier survival curves and multivariable Cox regression models were used to estimate the association of consumption of MedHi foods with mortality. Population-attributable fractions (PAFs) of consumption of MedHi foods in relation to mortality risk were also estimated.; RESULTS: A total of 35,299 adults aged≥20 years were included in this study. During a median follow-up of 9.67 years, compared with adults in G1, those in G3 had 16% (hazard ratio [HR], 0.84; 95% confidence interval [CI], 0.77-0.90) reduced risk of all-cause mortality, and 23% (HR, 0.77; 95% CI, 0.67-0.89) reduced risk of CVD-specific mortality. The PAF of high (G3) vs. intermediate or low consumption of MedHi foods (G1+G2) with all-cause and CVD-specific mortality was 3.4% and 4.3%, respectively.; CONCLUSIONS: Consumption of foods with higher microbial concentrations is associated with a reduced risk of all-cause and CVD-specific mortality in US adults. © 2024. Springer-Verlag GmbH Germany, part of Springer Nature.

Background: Dysfunction of endothelial cells links to microvascular rarefaction, reflecting the pathogenesis of hypertension. Our previous studies found that miR-3656 reduces nitric oxide generation and von Willebrand factor (vWF) cleavage, thereby retarding blood flow and potentially increasing blood pressure. In this paper, we investigated mechanism of transcription regulation contributing to miR3656-damaged endothelial cells in hypertension.Methods: The effects of miR-3656 on function of endothelial cells were analyzed on the basis of proliferation, migration, tube formation, and apoptosis. The mRNA level and protein level of genes were examined using quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. Dual-luciferase reporter assay was performed to confirm the binding between miR-3656 and 3 ' untranslated region (UTR) of transcription factor AP-2 gamma (TFAP2C). The binding between TFAP2C and the promoter region of Krppel-like factor 10 (KLF10) was confirmed by chromatin immunoprecipitation-qPCR assay.Results: miR-3656 impaired the cell proliferation, migration, tube formation, and apoptosis of endothelial cells. miR-3656 inhibited the expression of TFAP2C by directly targeting 3 ' UTR of TFAP2C; moreover, miR-3656induced injury of endothelial cells was rescued by TFAP2C overexpression. Furthermore, downregulated TFAP2C decreased KLF10 expression by binding to KLF10 promoter region, and upregulated KLF10 reversed the effects of silencing TFAP2C on endothelial cells. These inhibitory processes led to interference of miR-3656 to KLF10promoted function of endothelial cells. Conclusion: TFAP2C/KLF10 axis is involved in miR-3656related dysfunction of endothelial cells in hypertension. The 3 ' UTR of TFAP2C and KLF10 promoter region are the hubs of the TFAP2C/KLF10 axis.

微血管结构和功能改变是阻碍血流流速、形成高血压的主要原因。APOE基因中突变成无抗氧化能力氨基酸残基的基因多态性位点可能是高血压的危险因素。我前期发现:ApoE基因型(ε3/ε4和ε4/ε4)可能是高血压的危险因素。由于Cys残基数量的差别，从apoE2、apoE3到apoE4,呈现出明显下降的抗氧化能力，可见apoE2、apoE3和apoE4的功能存在差异。由于apoE无法穿透内皮细胞膜，我们推测apoE与内皮损伤之间可能存在可以穿过内皮细胞膜的中介分子。在本项目的资助下，我们发现miR-3656是apoE2、apoE3和apoE4富集的miR之一，富集程度差异明显,miR-3656是apoE4所致血管内皮损伤差异的中介分子:miR-3656通过靶向eNOS和ADAMTS13诱导血管内皮细胞损伤;miR-3656驱动的KLF10表达抑制是高血压内皮功能障碍的关键机制环节。我们进一步研究eNOS基因多态性与高血压的关系，发现eNOS rs1799983多态性与高血压风险增加相关。我们进行了长春地区高血压流行病学调查研究工作，发现长春市高血压的知晓率、治疗率和控制率均高于中国，说明长春市高血压防治工作是有效的。然而，中国老年人群中高血压的患病率低于长春，这也使得长春对高血压的监管面临巨大挑战。我们研究了老年人高血压前期或高血压向糖尿病发展的发病率及危险因素，发现与高血压前期参与者相比，高血压患者患糖尿病的风险较高。抗高血压药物与高血压患者从非糖尿病到糖尿病的趋势降低有关。同时，我们也研究了老成年居民多发病的社会地理和行为影响，发现高血压家族史和糖尿病家族史与高并发症风险显着相关。

BACKGROUND: Autism spectrum disorder (ASD) is a common neurodevelopmental disorder, with an increasing prevalence worldwide. Copy number variation (CNV), as one of genetic factors, is involved in ASD etiology. However, there exist substantial differences in terms of location and frequency of some CNVs in the general Asian population. Whole-genome studies of CNVs in Northeast Han Chinese samples are still lacking, necessitating our ongoing work to investigate the characteristics of CNVs in a Northeast Han Chinese population with clinically diagnosed ASD.; METHODS: We performed a genome-wide CNVs screening in Northeast Han Chinese individuals with ASD using array-based comparative genomic hybridization.; RESULTS: We found that 22 kinds of CNVs (6 deletions and 16 duplications) were potentially pathogenic. These CNVs were distributed in chromosome 1p36.33, 1p36.31, 1q42.13, 2p23.1-p22.3, 5p15.33, 5p15.33-p15.2, 7p22.3, 7p22.3-p22.2, 7q22.1-q22.2, 10q23.2-q23.31, 10q26.2-q26.3, 11p15.5, 11q25, 12p12.1-p11.23, 14q11.2, 15q13.3, 16p13.3, 16q21, 22q13.31-q13.33, and Xq12-q13.1. Additionally, we found 20 potential pathogenic genes of ASD in our population, including eight protein coding genes (six duplications [DRD4, HRAS, OPHN1, SHANK3, SLC6A3, and TSC2] and two deletions [CHRNA7 and PTEN]) and 12 microRNAs-coding genes (ten duplications [MIR202, MIR210, MIR3178, MIR339, MIR4516, MIR4717, MIR483, MIR675, MIR6821, and MIR940] and two deletions [MIR107 and MIR558]).; CONCLUSION: We identified CNVs and genes implicated in ASD risks, conferring perception to further reveal ASD etiology. © 2023. The Author(s).

Objectives To investigate the epidemiological status quo of hypertension in elderly population in Changchun, China, and provide a reference for the prevention and control strategies of hypertension of elderly population in this region. Design A cross-sectional study, as a part of a comprehensive project in Northeast China, was designed to perform in 10 districts in Changchun. Participants and setting A total of 6846 participants who were >= 60 years old were selected using a random sampling method. Main outcome measures The epidemiological status quo of hypertension. Results The prevalence of hypertension in Changchun was 52.6%. Among participants with hypertension enrolled in this study, 87.6% of the participants had been diagnosed with hypertension before the study, 69.1% was taking antihypertensive medications and 66.9% had effective blood pressure control. Obesity, widower/widow, history of diseases and family history of hypertension were risk factors of hypertension (all p<0.05). Participants with obesity, a personal history of heart coronary disease, or a family history of hypertension were susceptible to realising risks of hypertension (all p<0.05). However, participants with diabetes, hyperlipidaemia, or a family history of hypertension were difficult to control blood pressure within the normal range (all p<0.05). In addition, 92.6% participants taking antihypertensive medications used a single medication, and calcium channel blockers was the most commonly used antihypertensive medications in monotherapy. Conclusion The rates of awareness, treatment and control of hypertension are greater in Changchun than those in China, indicating that the prevention and control of hypertension in Changchun are effective. However, the prevalence of hypertension in the elderly population in China is lower than that in Changchun, also rendering Changchun a substantial challenge for the supervision of hypertension.