Background: The mechanism of a water-soluble extract of safflower on chronic alcoholic liver injury is still unclear. Objective: To study the mechanism of water extract of safflower on chronic alcoholic liver injury. Materials and Methods: The rats were separated in a random manner into five cohorts, namely, the normal control group (NC), alcoholic fatty liver disease model (ALD), ALD rat model treated with the water-soluble extract of safflower (30 g/kg), ALD rat model treated with the water-soluble extraction of safflower (40 g/kg), and ALD rat model with the water-soluble extract of safflower (50 g/kg). Rats in each group were anaesthetized with 2% isoflurane and euthanized by cervical dislocation after 1, 7, 14, 21, and 28 days treatment. Proteomic analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG analysis), immunohistochemistry (IHC), and western blot were employed for comparing the variations across model cohort and control cohort at the tissue level, protein level, and molecular level, respectively, and for probing potential prophylactic role/mechanisms for safflower extract on alcoholic liver injury. Results: KEGG analysis found that the differentially expressed genes in the alcoholic liver injury model were closely associated with alcoholism, inflammation, and serotonergic synapses. Furthermore, with the administration of water-soluble extract of safflower, these hepatic function indices and liver steatosis lesions were alleviated in both time and dose-dependent manners. Importantly, IHC analysis discovered that proteins disulfide isomerase A3 (PDIA3) and Trk-fused gene (TFG) were highly increased in the liver of the rats. Furthermore, the water-soluble extract of safflower alleviated liver oxidative injury and lipid peroxidation, via the upregulation of proteins such as 26S proteasome non-ATPase regulatory subunit 2 (PSMD2), cytochrome P450 2C6 (CYP2C6V1), and cytochrome P450 2C11 (CYP2C11), anddownregulation of arachidonate 12-lipoxygenase and 12S-type (ALOX12) protein expression. Conclusion: The water-soluble extract of safflower exhibited potential protective effects against lipid peroxidation and ethanol-driven oxidative damage within the liver via the upregulation of expression of proteins PSMD2, CYP2C6V1, and CYP2C11 and downregulation of the expression of ALOX12, PDIA3, and TFG proteins.

目的基于代谢组学探讨红花水提物治疗慢性酒精性肝损伤的作用机制。方法将大鼠按随机数字表法分为正常组、模型组、阳性对照组及红花水提物低、中、高剂量组,每组10只。除正常组外,其余各组灌胃56°牛栏山二锅头白酒8 ml/kg制备慢

目的探讨蒙药色日古德(长松萝)醇提物的正丁醇萃取成分(简称示例药物)对D-氨基半乳糖(D-GalN)中毒大鼠急性肝损伤的解毒作用机理。方法以D-GalN诱导建立大鼠急性肝损伤模型,观察示例药物对血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移

目的:研究蒙药长松萝的正丁醇萃取物化学成分。方法:应用多种色谱技术对长松萝进行分离纯化,并通过NMR等波谱方法鉴定化合物的结构。结果:从长松萝乙醇提取物的正丁醇萃取部位分离得到8个化合物,分别鉴定为:阿拉伯糖醇(Ⅰ)、邻苯二甲酸

目的初步探讨蒙药长松萝醇提物的石油醚、二氯甲烷、正丁醇、水层萃取成分对D-氨基半乳糖胺（D-Gal N）诱导的大鼠急性肝损伤的解毒作用机理。方法以D-Gal N诱导建立大鼠急性肝损伤模型,观察长松萝醇提物的石油醚、二氯甲烷、正丁醇萃取部位和剩余水层部位对血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）活性的作用变化。结果长松萝醇提物的正丁醇萃取部位有明显降低急性肝损伤大鼠血清ALT、AST活性,有效减轻D-Gal N所致的肝组织病理改变。结论蒙药长松萝醇提物的正丁醇萃取成分具有保肝解毒作用。