基于ERK/p38-eIF4E通路探讨PEDV抗病毒制剂的作用机理
项目来源
国（略）科（略）(（略）C（略）
项目主持人
王（略）
项目受资助机构
中（略）大（略）
项目编号
3（略）2（略）
立项年度
2（略）
立项时间
未（略）
研究期限
未（略） （略）
项目级别
国（略）
受资助金额
5（略）0（略）
学科
生（略）-（略）-（略）毒（略）
学科代码
C（略）8（略）8（略）
基金类别
面（略）
关键词
抗（略）制（略） （略）型（略）家（略） （略）药（略） （略）胞（略）统（略）p（略）i（略）;（略）t（略）r（略）d（略） （略）g（略）;（略）t（略）r（略）i（略）b（略）r（略）c（略）u（略） （略）n（略）t（略） （略）h（略）r（略） （略）m（略）m（略）l
参与者
李（略）韩（略）李（略）董（略）王（略）硕
参与机构
中（略）大（略）；河南农业大学；北京大学；北京理工大学
项目标书摘要：基于（略）研究抗病毒制剂，是（略）也是重要研究方向之（略）活ERK/p38-（略）化的eIF4E继而（略），利于病毒自身蛋白（略）毒PEDV给养猪业（略）特效防治药物。本课（略）现磷酸化eIF4E（略）碱HHT具有高效的（略）HT通过阻断病毒利（略）制病毒复制。本项目（略）用机理，主要内容包（略）eIF4E通路在P（略）嘌呤霉素标记、蛋白（略）究HHT如何靶向调（略）饰，选择性抑制病毒（略）用阶段以及体内外活（略）毒与细胞的互作关系（略）为开发仔猪病毒性腹（略）
Applicati（略）: Antivir（略）ased on h（略）rus inter（略）anisms ha（略） major re（略）st in the（略）irology i（略）few years（略）st utiliz（略）lar trans（略）inery to （略）al protei（略）fection c（略） ERK/p38-（略）ay;phosph（略）s mediate（略）pendent t（略）initiatio（略）pidemic d（略）onavirus(（略）verely th（略）ine indus（略）s no spec（略）vailable （略）atment of（略）tion.Base（略）nctional （略）ound homo（略）ne(HHT),w（略）s and deg（略）hospho-eI（略）y inhibit（略）lication.（略）sted that（略）ts eIF4E-（略）ranslatio（略） against （略）ulation.T（略） research（略）o exploit（略）ism in wh（略）ibits PED（略）.The spec（略）ives are （略）1).the ro（略）38-eIF4E （略）PEDV repl（略）.how the （略）es eIF4E （略）n to sele（略）ibit vira（略）on,by usi（略）n labelin（略）odificati（略） and regr（略）ing;(3).i（略） stage of（略）ction the（略）a role an（略）iral acti（略） in vitro（略）o.Results（略）roposed r（略）l not onl（略）mportant （略）knowledge（略）and the h（略）rus inter（略）will also（略）undation （略）elopment （略）l agents （略）V infecti（略）
项目受资助省
北（略）
项目结题报告(全文)
基于解析细胞—病毒（略），是病毒学领域重要（略）现高三尖杉酯碱HH（略）抑制子，具有高效的（略）抗病毒效果，动物实（略）HT与氯喹等联合用（略）感染过程中会激活E（略）E细胞通路，提高p（略）eIF4E有利于病（略）是抗病毒制剂的有效（略）了p-4E抑制剂R（略）抑制效果，以及基于（略）T定量蛋白质组筛选（略）p-eIF4E在病（略）步资料。本项目为开（略）供依据。
1.Selective regulation in ribosome biogenesis and protein production for efficient viral translation
- 关键词：
- Selective translation; Ribosome biogenesis factor; Ribosomal protein;Antiviral target;DEAD-BOX PROTEINS; MESSENGER-RNA; NUCLEOLAR LOCALIZATION;GENE-EXPRESSION; ENTRY SITE; O-GLCNAC; SPECIALIZED RIBOSOMES;CRYSTAL-STRUCTURE; NUCLEAR ANTIGEN; BINDING PROTEIN
As intracellular parasites, viruses depend heavily on host cell structures and their functions to complete their life cycle and produce new viral particles. Viruses utilize or modulate cellular translational machinery to achieve efficient replication; the role of ribosome biogenesis and protein synthesis in viral replication particularly highlights the importance of the ribosome quantity and/or quality in controlling viral protein synthesis. Recently reported studies have demonstrated that ribosome biogenesis factors (RBFs) and ribosomal proteins (RPs) act as multifaceted regulators in selective translation of viral transcripts. Here we summarize the recent literature on RBFs and RPs and their association with subcellular redistribution, post-translational modification, enzyme catalysis, and direct interaction with viral proteins. The advances described in this literature establish a rationale for targeting ribosome production and function in the design of the next generation of antiviral agents.
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3.Structural and molecular basis for foot-and-mouth disease virus neutralization by two potent protective antibodies
4.Selective regulation in ribosome biogenesis and protein production for efficient viral translation
- 关键词：
- Selective translation; Ribosome biogenesis factor; Ribosomal protein;Antiviral target;DEAD-BOX PROTEINS; MESSENGER-RNA; NUCLEOLAR LOCALIZATION;GENE-EXPRESSION; ENTRY SITE; O-GLCNAC; SPECIALIZED RIBOSOMES;CRYSTAL-STRUCTURE; NUCLEAR ANTIGEN; BINDING PROTEIN
As intracellular parasites, viruses depend heavily on host cell structures and their functions to complete their life cycle and produce new viral particles. Viruses utilize or modulate cellular translational machinery to achieve efficient replication; the role of ribosome biogenesis and protein synthesis in viral replication particularly highlights the importance of the ribosome quantity and/or quality in controlling viral protein synthesis. Recently reported studies have demonstrated that ribosome biogenesis factors (RBFs) and ribosomal proteins (RPs) act as multifaceted regulators in selective translation of viral transcripts. Here we summarize the recent literature on RBFs and RPs and their association with subcellular redistribution, post-translational modification, enzyme catalysis, and direct interaction with viral proteins. The advances described in this literature establish a rationale for targeting ribosome production and function in the design of the next generation of antiviral agents.
...5.Repurposing host-based therapeutics to control coronavirus and influenza virus
- 关键词：
- RESPIRATORY SYNDROME CORONAVIRUS; NF-KAPPA-B; DEPENDENT KINASEINHIBITOR; INFECTION IN-VITRO; A VIRUS; SARS CORONAVIRUS; TRANSLATIONINITIATION; ANTIVIRAL ACTIVITY; ENTERS CELLS; PROTEASE INHIBITORS
The development of highly effective antiviral agents has been a major objective in virology and pharmaceutics. Drug repositioning has emerged as a cost-effective and time-efficient alternative approach to traditional drug discovery and development. This new shift focuses on the repurposing of clinically approved drugs and promising preclinical drug candidates for the therapeutic development of host-based antiviral agents to control diseases caused by coronavirus and influenza virus. Host-based antiviral agents target host cellular machineries essential for viral infections or innate immune responses to interfere with viral pathogenesis. This review discusses current knowledge, prospective applications and challenges in the repurposing of clinically approved and preclinically studied drugs for newly indicated antiviral therapeutics.
...6.Repurposing host-based therapeutics to control coronavirus and influenza virus
- 关键词：
- RESPIRATORY SYNDROME CORONAVIRUS; NF-KAPPA-B; DEPENDENT KINASEINHIBITOR; INFECTION IN-VITRO; A VIRUS; SARS CORONAVIRUS; TRANSLATIONINITIATION; ANTIVIRAL ACTIVITY; ENTERS CELLS; PROTEASE INHIBITORS
The development of highly effective antiviral agents has been a major objective in virology and pharmaceutics. Drug repositioning has emerged as a cost-effective and time-efficient alternative approach to traditional drug discovery and development. This new shift focuses on the repurposing of clinically approved drugs and promising preclinical drug candidates for the therapeutic development of host-based antiviral agents to control diseases caused by coronavirus and influenza virus. Host-based antiviral agents target host cellular machineries essential for viral infections or innate immune responses to interfere with viral pathogenesis. This review discusses current knowledge, prospective applications and challenges in the repurposing of clinically approved and preclinically studied drugs for newly indicated antiviral therapeutics.
...