项目来源

国家自然科学基金(NSFC)

项目主持人

张成孝

项目受资助机构

陕西师范大学

立项年度

1999

立项时间

未公开

项目编号

29975017

项目级别

国家级

研究期限

未知 / 未知

受资助金额

12.00万元

学科

化学科学-化学测量学-电分析化学

学科代码

B-B04-B0402

基金类别

面上项目

关键词

化学发光检测 ; 氧化还原标记 ; 均相免疫分析 ; Homogeneous immunoassay.Redox label.Chemluminescence det

参与者

封满良；杨琨；王占玲；郑行望；高强；刘伟；郭志慧；金燕

参与机构

中国人民解放军第四军医大学

项目标书摘要：提出基于小分子氧化还原标记偶合化学发光检测的新型均相电化学催化化学发光免疫分析法。筛选新型小分子电催化化学发光免疫标记物，研究标记蛋白免疫反应特性和电化学发光特性，建立新型均相电化学催化化学发光免疫分析理论模式和快速简单高灵敏自动化均相电催化化学发光免疫分析方法。对生命科学和环境科学研究以及免疫分析的发展均具有重要的意义。

Application Abstract: The immunoassay is an front research subject of modern analytical chemistry that has been widely employed in life science and in environment science due to high specificity and high sensitivity.Electrogenerated chemiluminescence(ECL)immunoassay(ECLIA)is a promising analytical tool in immunossay.The project,"novel homogeneous ECL immunoassays",has focused on three research sections including the investigation of new ECL systems,electron transfer mediators and the development of homogeneous ECL immmunoassy.Two ECL methods of the determination of riboflavin and adrenson have been developed based on the ECL of lucigenin and Mn(Ⅲ),respectively.A novel flow injection analytical system with a screen-printed enzyme sensor incorporating Os-complex mediator was developed and a glucose amperometric sensor with an integrated pre-oxidation membrane to remove interference from ascorbic acid and uric acid was fabricated.Two novel homogeneous electrogenerated chemiluminescence(ECL)immunoassays for anti-digoxin antibody and digoxin hapten were developed based on a luminol-labeled digoxin hapten and a multilabeling of luminol and digoxin to a bovine serum albumin,respectively.Digoxin hapten was determined throughout its therapeutic range with a detection limit of 0.10 ng/ml and in a 3 orders of magnitude range.The proposed methods hvae been applied to assay anti-digoxin antibody and digoxin in human control serum with satisfactory results.The project achievement includes 4 research papers published on SCI journals,3 research papers on Chinese key journals and two presentations on 2002 Pittcon international conference and the goal of the project was mainly accomplished.The project achievements,homogeneous ECL immunoassay using luminuol-labeled small molecule hapten and multilabeling of luminol and digoxin,are promising alternative approach for improvement of the ECL quantum,ECL label ratio and the sensitivity of ECL immunoassay.The proposed ECL immunoassay for digoxin and anti-digoxin antibody is easily performed with high sensitivity and has a potential use in clinical assay and will promote the research work on the immunoassay in some aspects.

项目受资助省

陕西省

项目结题报告

新型均相电化学发光免疫分析法研究结题报告(全文)