项目来源

德国科学基金(DFG)

项目主持人

Dr. F. Jon Kull

项目受资助机构

未公开

项目编号

5175212

立项年度

1999

立项时间

未公开

项目级别

国家级

研究期限

未知 / 未知

受资助金额

未知

学科

未公开

学科代码

未公开

基金类别

Schwerpunktprogramme

关键词

未公开

参与者

Dr. F. Jon Kull

参与机构

未公开

项目标书摘要：The major focus of this project is to determine the mechanistic details of kinesin and myosin motor proteins through the use of X-ray crystallographic techniques. The catalytic motor domains of myosin will be overexpressed in Dictyostelium and both the motor domain as the entire kinesin protein and its light chains will be expressed in E. coli, as well as Dictyostelium expression systems. A number of mutant and modified myosin II motor fragments are already available for structural analysis and the expression level, purity, and quality of crystals obtained from the these soluble constructs will be optimized. Together with Dr. D. J. Manstein (MPI for Medical Research), the structural characterization of Class I, II, V, and VII myosin proteins will be attempted. Additionally, in collaboration with Dr. D. J. Manstein, Dr. I. Schlichting (MPI Dortmund) and H. G. Mannherz (Ruhr University Bochum), we will attempt to determine the high resolution structure of the myosin motor in complex with actin.