项目来源

国家重点研发计划(NKRD)

项目主持人

牟峰

项目受资助机构

深圳华大智造科技股份有限公司

立项年度

2022

立项时间

未公开

项目编号

2022YFF1202201

项目级别

国家级

研究期限

未知 / 未知

受资助金额

0.52万元

学科

生物与信息融合(BT与IT融合)

学科代码

未公开

基金类别

“生物与信息融合”重点专项

关键词

便携测序仪 ; 半导体测序仪 ; 生物自发光 ; 微流控芯片 ; Portable sequencer ; semiconductor based sequencer ; bioluminescence ; microfluidic chip

参与者

何琳；史兴；黄恒；黄扶兴；谢伟伟

参与机构

青岛华大智造科技有限责任公司

项目标书摘要：本课题拟研发一套便携易用的半导体测序系统，其信号产生方式为生物自发光，图像传感器半导体芯片直接信号读取到仪器内部电路中，使仪器实现“无光学系统”。同时拟开展多核心封装技术研究，并通过像元仿真和测序实验，提升像元密度，实现有效 reads 数的大幅提升。本课题还将研究其表面图案化刻蚀及表面修饰技术，高效率地实现 DNA 纳米球和像元的一一对应装载，实现芯片上直接生化反应。测序仪的流体系统拟通过集成微米级沟道和多路选通阀的微流控芯片实现，开发高分子聚合物流体芯片和硅基半导体芯片及其载板的集成封装技术。同步开发适应 POCT 形态的快速装载集成试剂盒。本报告从测序载片开发、测序试剂盒开发以及测序仪器开发三个维度，阐述了一年以来取得的进展:测序载片方面：完成612nm像素尺寸的≥100M通量第三种半导体集成电路测序载片光学仿真；完成≥25M,≥100M所需传感器封装基板设计与打样、样品封装、信号读取与调试；完成2颗核心传感器封装的≥25M通量测序载片同微流体芯片合封成品开发。测序试剂盒方面：完成无需光机系统生物自发光的单端200bp,双端 100bp基因测序可行性;SE100和PE150两种快速装载集成基因测序试剂盒的小试和中试开发;测序仪器方面：完成“无光学系统”的小型化、低功耗基因测序仪整机开发和转产。并带有一体式生信分析计算模块，实现从待测样本到报告快速生成。

Application Abstract: This project plans to develop a portable and easy-to-use semiconductor sequencing system.The signal generation method is biological self-luminescence.The image sensor semiconductor chip directly reads the signal into the internal circuit of the instrument,making the instrument"no optical system".At the same time,it is planned to carry out research on multi-core packaging technology,and increase the pixel density through pixel simulation and sequencing experiments to achieve a significant increase in the number of effective reads.This topic will also study its surface patterning etching and surface modification technology to efficiently achieve one-to-one loading of DNA nanospheres and pixels to achieve direct biochemical reactions on the chip.The fluidic system of the sequencer is planned to be realized through a microfluidic chip integrating micron-scale channels and multi-way gate valves,and integrated packaging technology for polymer fluidic chips and silicon-based semiconductor chips and their carrier boards will be developed.Simultaneously develop a fast-loading integrated kit adapted to POCT morphology.This report explains the progress made over the past year from three dimensions:sequencing slide development,sequencing kit development,and sequencing instrument development:In terms of sequencing slides:complete the optical simulation of the third semiconductor integrated circuit sequencing slide with 612nm pixel size and ≥100M throughput;complete the design and proofing of sensor packaging substrates required for ≥25M and ≥100M,sample packaging,signal reading and debugging;Completed the development of a ≥25M throughput sequencing slide packaged with 2 core sensors and a microfluidic chip packaged product.In terms of sequencing kits:Complete the feasibility of single-end 200bp and double-end 100bp gene sequencing that does not require bioautoluminescence of an opto-mechanical system;develop small-scale and pilot-scale trials of two fast-loading integrated gene sequencing kits,SE100 and PE150;In terms of sequencing instruments:Complete the development and production conversion of a miniaturized,low-power gene sequencer without optical systems.It also comes with an integrated biochemical analysis and calculation module to quickly generate reports from samples to be tested.

项目受资助省

广东省