项目来源

湖北省自然科学基金

项目主持人

李大主要

项目受资助机构

华中科技大学

立项年度

2015

立项时间

未公开

项目编号

2015CFA073

项目级别

省级

研究期限

未知 / 未知

受资助金额

10.00万元

学科

人口与健康生物医药

学科代码

未公开

基金类别

未公开

关键词

TSLPR抗体 ; 动脉粥样硬化 ; 动脉粥样硬化血栓形成 ; 血小板 ; TSLPR Antibody ; Atherosclerosis ; Atherothrombosis ; Platelet

参与者

李江华

参与机构

未公开

项目标书摘要：目的：探讨TSLPR抗体在抑制动脉粥样硬化血栓形成中的作用。方法:8周龄载脂蛋白E-/-小鼠，给与高脂饲养 12周，期间分别周期性尾静脉注射TSLPR抗体和同型无关IgG抗体，检测各组小鼠动脉粥样硬化血栓形成时间，血小板聚集和释放功能，血小板膜糖蛋白表达。结果：与对照组相比,TSLPR抗体处理组血栓形成时间明显长，血小板聚集功能和释放功能明显低，血小板活化指标CD62p、CD63、pac-1表达明显低，静息指标CD42b表达明显高。结论：静脉注射TSLPR抗体可以明显抑制血小板聚集功能和释放功能，降低血小板活化程度，从而抑制动脉粥样硬化血栓形成。

Application Abstract: AIM:To explore the role of TSLPR antibody in the atherothrombosis inhibition.METHODS:8 weeks old ApoE-/-mice were fed with high-cholesterol diet for 12 weeks.During this time,mice in different group were injected periodically with TSLPR antibody or isotype IgG respectively.The time of atherothrombosis,platelet aggregation function,platelet release function,and the expression of platelet membrane glycoprotein in different groups were detected.Results:Compared with those in the IgG control group,in the TSLPR antibody group the time of atherothrombosis was prolonged,the function of platelet aggregation and release was weakened,the expression of CD62p,CD63,pac-1 was decreased,the expression of CD42b was increased.Conclusion:TSLPR antibody injection could inhibit atherosclerosis thrombus formation via weakening platelet aggregation and release function and repressing platelet activation.

项目受资助省

湖北省