主要农作物养分高效利用性状形成的遗传与分子基础
项目来源
国（略）研（略）(（略）D（略）
项目主持人
徐（略）
项目受资助机构
中（略）大（略）
立项年度
2（略）
立项时间
未（略）
项目编号
2（略） YFD0100700
项目级别
国（略）
研究期限
未（略） （略）
受资助金额
9（略）0（略）
学科
七（略）物（略）
学科代码
未（略）
基金类别
“七（略）物（略）”重点专项
关键词
玉（略） （略）;（略）高（略） （略）运（略）;（略）变（略） （略）组（略）析（略）养（略）品（略） （略）z（略） （略）e（略）d（略）n（略）i（略） （略） （略）i（略）n（略）;（略）t（略）n（略）r（略）p（略）e（略）;（略）l（略）c（略）r（略）i（略）;（略）n（略）-（略）e（略）s（略）a（略）n（略）n（略）i（略）-（略） （略）i（略）n（略）u（略）v（略）
参与者
袁（略）徐（略）高（略）陈（略）李（略）王（略）益（略）
参与机构
华（略）大（略）川（略）学
项目标书摘要：本课（略）率遗传改良的瓶颈问（略）、遗传学、基因组学（略）在挖掘一批氮、磷、（略）基因及其优良等位变（略）的优异种质材料。主（略）)利用多个氮磷钾养（略）间筛选与评价近千份（略）定得到一批养分高效（略）利用全基因组关联分（略）玉米氮、磷、钾养分（略）状显著关联的关键候（略）阐明玉米养分高效吸（略）酸盐转运蛋白基因Z（略）系硝态氮的吸收及信（略）双加氧酶基因ZmC（略）地上部的运输；磷转（略）其互作蛋白激酶Zm（略）和老叶—新叶磷的再（略）系钾高效吸收的钾转（略）;鉴定到调控玉米地（略）转录因子基因ZmR（略）夹角性状的生长素响（略）0。3)遗传改良高（略）亲本，育成氮高效玉（略）UE25,组配杂交（略）%和节氮18%。4（略）因BnaA3.NI（略）NIP5;1协同调（略）aC4.BOR1;（略）载，以及硼向地上部（略）。发掘与利用Bna（略）良等位变异，创制2（略）究成果为培育养分高（略）提供优异基因资源和（略）
Applicati（略）: To addr（略） question（略）ng nutrie（略）ciency in（略）mays)and （略）rassica n（略）ing combi（略）hes on pl（略）on,geneti（略）cular bio（略）roject ai（略）tify key （略）heir favo（略）s involve（略） and util（略）nitrogen(（略）us(P),pot（略）nd furthe（略）rient-use（略）germplasm（略）results a（略）ons are p（略） follows:（略） of nutri（略）icient ma（略）lines wer（略）d by scre（略） 1,000 li（略）ral field（略）h long-te（略）ation exp（略）me key ge（略）genes and（略）ral varia（略）iated wit（略） roots an（略） use effi（略） identifi（略）e-wide as（略）nalysis a（略）S).(2)We （略）he roles （略）transport（略）RT1.1B in（略） nitrate （略）signaling（略） roots;th（略） of Carot（略）age Dioxy（略） ZmCCD10a（略）ing root-（略）transloca（略）les of ph（略）nsporter （略）and its u（略）ulator,a （略）ase ZmCK2（略）ng P upta（略） and P mo（略）from old （略）oung leav（略）tion of p（略）ansporter（略）5 on upta（略） the impr（略）K use eff（略） roles of（略）nscriptio（略）ne,ZmRAP2（略）ating bra（略）elopment （略）n respons（略）A10 invol（略）ts of roo（略）By geneti（略） the pare（略）of modern（略）id XY335,（略）ize inbre（略）NUE12 and（略） high N u（略）cy were b（略）e corresp（略）id NE30 w（略）d.By fiel（略）aluation （略）ars,NE30 （略）er perfor（略） a reduct（略）n N ferti（略）cation an（略） 3%on gra（略）)Identifi（略）character（略）boron(B)t（略） genes,Bn（略）and BnaA2（略）regulatin（略）and BnaC4（略） loading （略）m and tra（略）B to repr（略）gans.The （略） allele o（略）5;1Q was （略）ntified a（略）develop t（略） lines wi（略）se effici（略） together（略）ings high（略） novel me（略） nutrient（略） utilizat（略）s,and pro（略）le genes （略）sm resour（略）eding hig（略）use effic（略）and rapes（略）rs.
项目受资助省
北（略）
1.Genomic basis determining root system architecture in maize.
- Li, Pengcheng;Zhang, Zhihai;Xiao, Gui;Zhao, Zheng;He, Kunhui;Yang, Xiaohong;Pan, Qingchun;Mi, Guohua;Jia, Zhongtao;Yan, Jianbing;Chen, Fanjun;Yuan, Lixing
- 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》
- 2024年
- 137卷
- 5期
- 期刊
KEY MESSAGE: A total of 389 and 344 QTLs were identified by GWAS and QTL mapping explaining accumulatively 32.2-65.0% and 23.7-63.4% of phenotypic variation for 14 shoot-borne root traits using more than 1300 individuals across multiple field trails. Efficient nutrient and water acquisition from soils depends on the root system architecture (RSA). However, the genetic determinants underlying RSA in maize remain largely unexplored. In this study, we conducted a comprehensive genetic analysis for 14 shoot-borne root traits using 513 inbred lines and 800 individuals from four recombinant inbred line (RIL) populations at the mature stage across multiple field trails. Our analysis revealed substantial phenotypic variation for these 14 root traits, with a total of 389 and 344 QTLs identified through genome-wide association analysis (GWAS) and linkage analysis, respectively. These QTLs collectively explained 32.2-65.0% and 23.7-63.4% of the trait variation within each population. Several a priori candidate genes involved in auxin and cytokinin signaling pathways, such as IAA26, ARF2, LBD37 and CKX3, were found to co-localize with these loci. In addition, a total of 69 transcription factors (TFs) from 27 TF families (MYB, NAC, bZIP, bHLH and WRKY) were found for shoot-borne root traits. A total of 19 genes including PIN3, LBD15, IAA32, IAA38 and ARR12 and 19 GWAS signals were overlapped with selective sweeps. Further, significant additive effects were found for root traits, and pyramiding the favorable alleles could enhance maize root development. These findings could contribute to understand the genetic basis of root development and evolution, and provided an important genetic resource for the genetic improvement of root traits in maize. © 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
...2.Comparative functional analyses of PHR1, PHL1, and PHL4 transcription factors in regulating Arabidopsis responses to phosphate starvation
- 关键词：
- PHR1; PHL1; PHL4; Pi starvation responses; flowering time;transcriptomic analyses; functional relationship;GENE; EXPRESSION; HOMEOSTASIS; PHO1; TOLERANCE; THALIANA
- Wang, Zhen;Zheng, Zai;Liu, Dong
- 《FRONTIERS IN PLANT SCIENCE》
- 2024年
- 15卷
- 期
- 期刊
To cope with phosphate (Pi) starvation, plants trigger an array of adaptive responses to sustain their growth and development. These responses are largely controlled at transcriptional levels. In Arabidopsis (Arabidopsis thaliana), PHOSPHATE RESPONSE 1 (PHR1) is a key regulator of plant physiological and transcriptional responses to Pi starvation. PHR1 belongs to a MYB-CC-type transcription factor family which contains 15 members. In this PHR1 family, PHR1/PHR1-like 1(PHL1) and PHL2/PHL3 form two distinct modules in regulating plant development and transcriptional responses to Pi starvation. PHL4 is the most closely related member to PHR1. Previously, using the phr1phl4 mutant, we showed that PHL4 is also involved in regulating plant Pi responses. However, the precise roles of PHL1 and PHL4 in regulating plant Pi responses and their functional relationships with PHR1 have not been clearly defined. In this work, we further used the phl1phl4 and phr1phl1phl4 mutants to perform comparative phenotypic and transcriptomic analyses with phr1, phr1phl1, and phr1phl4. The results showed that both PHL1 and PHL4 act redundantly and equally with PHR1 to regulate leaf senescence, Pi starvation induced-inhibition of primary root growth, and accumulation of anthocyanins in shoots. Unlike PHR1 and PHL1, however, the role of PHL4 in maintaining Pi homeostasis is negligible. In regulating transcriptional responses to Pi starvation at genomic levels, both PHL1 and PHL4 play minor roles when acts alone, however, they act synergistically with PHR1. In regulating Pi starvation-responsive genes, PHL4 also function less than PHL1 in terms of the number of the genes it regulates and the magnitude of gene transcription it affects. Furthermore, no synergistic interaction was found between PHL1 and PHL4 in regulating plant response to Pi starvation. Therefore, our results clarified the roles of PHL1 and PHL4 in regulating plant responses to Pi starvation. In addition, this work revealed a new function of these three transcription factors in regulating flowering time.
...3.Functions and regulation of phosphate starvation-induced secreted acid phosphatases in higher plants
- 关键词：
- Phosphate starvation; Secreted acid phosphatases; Function; Regulation;Phosphate signaling;INDUCED GENE-EXPRESSION; ARABIDOPSIS-THALIANA; MOLECULARCHARACTERIZATION; PHOSPHORUS-DEFICIENCY; TRANSCRIPTIONAL RESPONSES;TRANSGENIC EXPRESSION; SUSPENSION CELLS; ROOTS; ATPAP26; ETHYLENE
Phosphorus is essential for plant growth and development, but levels of inorganic phosphate (Pi), the major form of phosphorus that plants assimilate, are quite limiting in most soils. To cope with Pi deficiency, plants trigger a suite of adaptive responses, including the induction and secretion of acid phosphatases (APases). In this article, we describe how Pi starvation-induced (PSI) APases are analyzed, and we provide a brief historical review of their identification. We then discuss the current understanding of the functions of PSI-secreted APases and how these APases are regulated at the molecular level. Finally, we provide a perspective on the future direction of research in this field.
...4. Single-cell RNA sequencing identifies a population of human liver-type ILC1s.Cell Reports,Vol.42.2023,Issue 1,111937.
5.Overexpression of AHL proteins enhances root hair production by altering the transcription of RHD6-downstream genes
- 关键词：
- AHL proteins; Arabidopsis thaliana; gene transcription; HSP70;protein-protein interaction; regulatory mechanism; RHD6; root hairdevelopment;AT-HOOK PROTEIN; CYTOSOLIC/NUCLEAR HSC70; MOLECULAR CHAPERONES;REGULATORY NETWORK; MEDIATOR COMPLEX; HYPOCOTYL GROWTH; PLANT MEDIATOR;ARABIDOPSIS; RESPONSES; HSP70
- Zeng, Qike;Song, Li;Xia, Mingzhe;Zheng, Zai;Chen, Ziang;Che, Ximing;Liu, Dong
- 《PLANT DIRECT》
- 2023年
- 7卷
- 8期
- 期刊
AT-HOOK MOTIF NUCLEAR LOCALIZED (AHL) proteins occur in all sequenced plant species. They bind to the AT-rich DNA sequences in chromosomes and regulate gene transcription related to diverse biological processes. However, the molecular mechanism underlying how AHL proteins regulate gene transcription is poorly understood. In this research, we used root hair production as a readout to study the function of two Arabidopsis AHL proteins, AHL17, and its closest homolog AHL28. Overexpression of AHL17 or AHL28 greatly enhanced root hair production by increasing the transcription of an array of genes downstream of RHD6. RHD6 is a key transcription factor that regulates root hair development. Mutation of RHD6 completely suppressed the overproduction of root hairs by blocking the transcription of AHL17-activated genes. The overexpression of AHL17 or AHL28, however, neither affected the transcription of RHD6 nor the accumulation of RHD6 protein. These two AHL proteins also did not directly interact with RHD6. Furthermore, we found that three members of the Heat Shock Protein70 family, which have been annotated as the subunits of the plant Mediator complex, could form a complex with both AHL17 and RHD6. Our research might reveal a previously unrecognized mechanism of how AHL proteins regulate gene transcription.
...6. Type: Other Status: Published Year Published: 2015 Citation: Majumdar, A. 2011, 2013, 2015. Home Garden Vegetables: Insect Control. ANR-1305. Alabama Cooperative Extension System, Auburn University. Circulation: 1,500. [On-line] http://www.aces.edu/pubs/docs/I/IPM-1305/IPM-1305.pdf
7. Type: Journal Articles Status: Published Year Published: 2016 Citation: Wurtzel, E.T., and Kutchan T.M. 2016. Plant metabolism, the diverse chemistry set of the future. Science 353: 1232-1236
8.磷酸乙醇胺N-甲基转移酶PMT1调控盐胁迫下拟南芥主根伸长的分子机制探究
- 关键词：
- 盐胁迫;根系发育;磷酸乙醇胺N-甲基转移酶;脱落酸;活性氧
- 何琦瑜
- 指导老师：浙江大学 杨建立
- 0年
- 学位论文
土壤盐渍化是当前最主要的环境问题之一,它会给植物带来离子毒害、渗透胁迫以及一系列的次生效应,例如氧化胁迫,导致植物生长抑制,作物产量降低。而揭示植物的耐盐机制能为培育耐盐新作物提供理论基础。本研究筛选到一个盐敏感的T-DNA插入突变体SALK＿108751,在盐胁迫下表现为主根伸长抑制、分生区长度缩短以及过渡区和伸长区出现肿胀形态的表皮细胞。利用CRISPR/Cas9基因编辑技术和回补实验,确定了磷酸乙醇胺N-甲基转移酶PMT1的突变是导致突变体对盐胁迫敏感的原因。利用遗传学手段,结合一系列生理生化实验,初步揭示了PMT1参与植物耐盐的分子机制,主要的研究结果如下:1.基因表达分析表明PMT1在根中的表达显著高于PMT2和PMT3,其中PMT1和PMT2都能受Na Cl诱导上调,且PMT1的上调更为明显,PMT3的表达受Na Cl处理抑制。盐敏感分析试验发现pmt1而非pmt2或pmt3单突变体对盐胁迫敏感。双突中,pmt1 pmt2在盐胁迫下表型与pmt1单突相近,pmt2 pmt3对盐处理不敏感,pmt1 pmt3存在发育缺陷,依据现有的证据,我们认为不同PMTs在盐胁迫响应过程中功能不冗余,并且PMT1是拟南芥幼苗中参与调控盐胁迫下主根伸长的关键磷酸乙醇胺N-甲基转移酶。2.盐胁迫下,pmt1根分生区长度缩短,细胞数目减少,通过引入CYCB1;1:GUS和DR5:GUS报告株系到pmt1和WT中,我们发现,盐胁迫抑制了根分生区细胞的分裂,改变了根对生长素的响应。且ABA处理也能模拟这种现象。表明Na Cl以及ABA对主根发育的调控都依赖于PMT1介导的分生区活性的维持。3.离子含量测定结果显示,pmt1在盐胁迫下主根伸长抑制并非由K+/Na+比的改变所导致的。进一步进行甘油脂含量测定。数据表明,在不含Na Cl的培养基生长,pmt1根中仅磷脂酰乙醇胺的丰度高于WT。在含有Na Cl的培养基生长后,pmt1根中磷脂酰胆碱和单半乳糖二酰甘油的含量都比WT低,但三酰甘油和磷脂酸的含量比WT高,表明盐胁迫改变了pmt1根中甘油脂的代谢。外源添加大豆卵磷脂能恢复突变体在盐胁迫下根短的表型。可见,pmt1的主根伸长与PMT1依赖的甘油脂的代谢相关。4.通过JASPAR在线软件分析,我们发现PMT1的启动子中存在ABA响应元件（ABREs）,且外源ABA能够诱导PMT1的表达。而在突变体aba2-1及pyl112458-T中,这种调控减弱甚至完全消失,表明Na Cl调控PMT1的表达依赖于ABA信号途径。5.pmt1突变体的主根对外源ABA敏感,单在pmt1的背景下敲除ABA2能有效缓解盐胁迫下pmt1根伸长的抑制以及表皮细胞不规则的凸起。表明盐胁迫下pmt1的根伸长受到抑制和外表皮细胞肿胀与ABA相关。6.NBT和DAB显色表明,Na Cl处理导致pmt1根中柱有更多的H2O2和O2-的积累,并且H2O2在pmt1根分生区的面积缩小。然而外源添加GSH并不能明显缓解pmt1在盐胁迫下的主根受到抑制的表型,表明盐胁迫导致pmt1根尖ROS的分布更可能是造成主根发育受阻的原因。并且,盐胁迫下,pmt1-2 aba2-1中ROS的积累和分布更偏向于WT或aba2-1而非pmt1-2,表明ABA可以通过调节pmt1中ROS的稳态并影响根系发育。综上,盐胁迫依赖ABA信号调控PMT1的转录,PMT1通过影响细胞分裂,生长素分布,甘油脂代谢等调控盐胁迫下根系发育,且PMT1依赖的脂质合成能够缓解盐胁迫下ABA诱导的活性氧的爆发,从而维持细胞膜的完整和主根伸长。
...9.A novel secreted protein, NISP1, is phosphorylated by soybean Nodulation Receptor Kinase to promote nodule symbiosis
- 关键词：
- legume-rhizobia symbiosis; Nodulation Receptor Kinase; proteinphosphorylation; root nodule symbiosis; soybean;SMALL-PEPTIDE SIGNALS; SYMRK; INTERACTS; INFECTION; BINDING; NUMBER;LIGASE; GENES
- Fu, Baolan;Xu, Zhipeng;Lei, Yutao;Dong, Ru;Wang, Yanan;Guo, Xiaoli;Zhu, Hui;Cao, Yangrong;Yan, Zhe
- 《JOURNAL OF INTEGRATIVE PLANT BIOLOGY》
- 2023年
- 卷
- 期
- 期刊
Nodulation Receptor Kinase (NORK) functions as a co-receptor of Nod factor receptors to mediate rhizobial symbiosis in legumes, but its direct phosphorylation substrates that positively mediate root nodulation remain to be fully identified. Here, we identified a GmNORK-Interacting Small Protein (GmNISP1) that functions as a phosphorylation target of GmNORK to promote soybean nodulation. GmNORK alpha directly interacted with and phosphorylated GmNISP1. Transcription of GmNISP1 was strongly induced after rhizobial infection in soybean roots and nodules. GmNISP1 encodes a peptide containing 90 amino acids with a "DY" consensus motif at its N-terminus. GmNISP1 protein was detected to be present in the apoplastic space. Phosphorylation of GmNISP1 by GmNORK alpha could enhance its secretion into the apoplast. Pretreatment with either purified GmNISP1 or phosphorylation-mimic GmNISP1(12D) on the roots could significantly increase nodule numbers compared with the treatment with phosphorylation-inactive GmNISP1(12A). The data suggested a model that soybean GmNORK phosphorylates GmNISP1 to promote its secretion into the apoplast, which might function as a potential peptide hormone to promote root nodulation.
...10.水稻抽穗期分子调控研究进展
- 关键词：
- 水稻（Oryza sativa） 抽穗期 基因 分子机制 基金资助：国家自然科学基金（31800238）； 国家重点研发计划（2016YFD0100706）； 重庆市教委科学技术研究项目（KJ1600310）共同资助； DOI：10.13271/j.mpb.017.007071 专辑：农业科技 专题：农作物 分类号：S511 手机阅读
- 蒋丹;洪广成;陈倩;刘石锋;秦小健
- 0年
- 卷
- 期
- 期刊
水稻作为人类最主要的粮食作物之一,其稳产高产与粮食安全和经济发展密切相关。抽穗期作为水稻的重要农艺性状,涉及的相关基因较多,基因之间的相互调控网络也较复杂。本研究对目前已克隆的水稻抽穗期相关调控基因进行梳理,分析了Hd1、Hd3a、OsGI、Ehd3、Ghd7、RFT1和Ehd1等基因的结构及功能。研究发现,这些基因主要通过Hd1-Hd3a和Ghd7-Ehd1-Hd3a/RFT1两条通路对水稻抽穗期进行调控,为水稻抽穗期育种和农业生产提供科学依据。
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